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Literature summary extracted from

  • Begara-Morales, J.C.; Sanchez-Calvo, B.; Gomez-Rodriguez, M.V.; Chaki, M.; Valderrama, R.; Mata-Perez, C.; Lopez-Jaramillo, J.; Corpas, F.J.; Barroso, J.B.
    Short-term low temperature induces nitro-oxidative stress that deregulates the NADP-Malic enzyme function by tyrosine nitration in Arabidopsis thaliana (2019), Antioxidants (Basel), 8, 448 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 peroxynitrite peroxynitrite inhibits cytosolic NADP-ME2 activity due to tyrosine nitration at Tyr-73 to 3-nitrotyrosine Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Arabidopsis thaliana
-
 pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+ Arabidopsis thaliana
-
 (S)-malate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Arabidopsis thaliana
-
 ecotype Columbia
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.40 nitration Tyr-73 of the enzyme is exclusively nitrated to 3-nitrotyrosine by peroxynitrite Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 LY-TRAP column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 leaf
-
 Arabidopsis thaliana
-
1.1.1.40 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Arabidopsis thaliana pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+
-
 Arabidopsis thaliana (S)-malate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 ? x * 84300, LY-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 NADP-malate dehydrogenase
-
 Arabidopsis thaliana
1.1.1.40 NADP-malic enzyme
-
 Arabidopsis thaliana
1.1.1.40 NADP-ME2 isoform Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.1.1.40 Arabidopsis thaliana 25% diminished cytosolic enzyme activity is found in Arabidopsis thaliana seedlings exposed to short-term low temperature (4°C for 48 h) down