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Literature summary extracted from

  • Zhao, S.; Liu, L.
    Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 (2016), Acta Crystallogr. Sect. F, 72, 795-798 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.13 ligand-free enzyme, sitting drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M lithium sulfate. Enzyme bound to GTP and D-mannose 1-phosphate, sitting drop vapor diffusion method, using 0.1 M magnesium formate, 15% (w/v) PEG 3350 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ activates Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Arabidopsis thaliana
-
diphosphate + GDP-D-mannose
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Arabidopsis thaliana O22287
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 Ni-NTA column chromatography, and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
Arabidopsis thaliana diphosphate + GDP-D-mannose
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 ? x * 45000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 CYT1
-
Arabidopsis thaliana
2.7.7.13 GDP-D-mannose pyrophosphorylase
-
Arabidopsis thaliana
2.7.7.13 GMPP
-
Arabidopsis thaliana
2.7.7.13 VTC1 gene name, vitamin C defective 1 Arabidopsis thaliana