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Literature summary extracted from

  • Bose, S.; Purkait, D.; Joseph, D.; Nayak, V.; Subramanian, R.
    Structural and functional characterization of CMP-N-acetylneuraminate synthetase from Vibrio cholerae (2019), Acta Crystallogr. Sect. D, 75, 564-577 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.43 expressed in Escherichia coli Rosetta 2 DE3 cells Vibrio cholerae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.43 apoenzyme, hanging drop vapor diffusion method, using 200 mM calcium acetate, 0.1 M imidazole pH 8.0, 10% (w/v) PEG 8000 Vibrio cholerae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.08
-
 CTP at pH 7.5 and 25°C Vibrio cholerae
2.7.7.43 0.127
-
 N-acylneuraminate at pH 7.5 and 25°C Vibrio cholerae
2.7.7.43 1.483
-
 N-glycolneuraminate at pH 7.5 and 25°C Vibrio cholerae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Mg2+ required Vibrio cholerae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate Vibrio cholerae
-
 diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolneuraminate Vibrio cholerae N-glycolneuraminate is a relatively weaker substrate than N-acylneuraminate diphosphate + CMP-N-glycolneuraminate
-
 ?
2.7.7.43 CTP + sialic acid Vibrio cholerae
-
 CMP-sialic acid + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Vibrio cholerae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43 affinity chromatography and gel filtration Vibrio cholerae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Vibrio cholerae diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolneuraminate N-glycolneuraminate is a relatively weaker substrate than N-acylneuraminate Vibrio cholerae diphosphate + CMP-N-glycolneuraminate
-
 ?
2.7.7.43 CTP + sialic acid
-
 Vibrio cholerae CMP-sialic acid + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.43 dimer
-
 Vibrio cholerae

Synonyms

EC Number Synonyms Comment Organism
2.7.7.43 CMAS
-
 Vibrio cholerae
2.7.7.43 CMP-N-acetylneuraminate synthetase
-
 Vibrio cholerae
2.7.7.43 CMP-sialic acid synthetase
-
 Vibrio cholerae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.43 2.21
-
 N-glycolneuraminate at pH 7.5 and 25°C Vibrio cholerae
2.7.7.43 3.97
-
 CTP at pH 7.5 and 25°C Vibrio cholerae
2.7.7.43 4.46
-
 N-acylneuraminate at pH 7.5 and 25°C Vibrio cholerae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.43 1.5
-
 N-glycolneuraminate at pH 7.5 and 25°C Vibrio cholerae
2.7.7.43 55.75
-
 N-acylneuraminate at pH 7.5 and 25°C Vibrio cholerae