EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.191 | expressed in Escherichia coli BL21(DE3) cells | Clostridioides difficile |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.191 | 0.576 | - |
GDP-D-mannose | at pH 8.0 and 37°C | Clostridioides difficile | |
2.7.1.191 | 0.934 | - |
Glcbeta1,3-GalNAc-PP-CPh | at pH 8.0 and 37°C | Clostridioides difficile |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.191 | Clostridioides difficile | - |
- |
- |
2.7.1.191 | Clostridioides difficile 630 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.191 | Ni-NTA column chromatography | Clostridioides difficile |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.191 | -20°C, 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% (v/v) glycerol, 3 months, no loss of activity | Clostridioides difficile |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.191 | GDP-2-azido-2-deoxy-D-glucose + Glcbeta1,3-GalNAc-PP-CPh | 61% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-2-azido-2-deoxy-D-glucose + Glcbeta1,3-GalNAc-PP-CPh | 61% activity compared to GDP-D-mannose | Clostridioides difficile 630 | ? | - |
? | |
2.7.1.191 | GDP-2-azido-2-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 86% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-2-azido-2-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 86% activity compared to GDP-D-mannose | Clostridioides difficile 630 | ? | - |
? | |
2.7.1.191 | GDP-4-azido-4-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 31% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-4-azido-4-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 31% activity compared to GDP-D-mannose | Clostridioides difficile 630 | ? | - |
? | |
2.7.1.191 | GDP-D-glucopyranosylamine + Glcbeta1,3-GalNAc-PP-CPh | 67% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-D-glucose + Glcbeta1,3-GalNAc-PP-CPh | 66% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-D-mannobiosyl fluoride + Glcbeta1,3-GalNAc-PP-CPh | 88% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-D-mannopyranosylamine + Glcbeta1,3-GalNAc-PP-CPh | 64% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | GDP-D-mannose + Glcbeta1,3-GalNAc-PEG | - |
Clostridioides difficile | Man-alpha1-P-6-Glc-beta1,3-GalNAc-PEG + GMP | - |
? | |
2.7.1.191 | GDP-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 100% activity | Clostridioides difficile | Man-alpha1-P-6-Glc-beta1,3-GalNAc-PP-CPh + GMP | - |
? | |
2.7.1.191 | GDP-D-talose + Glcbeta1,3-GalNAc-PP-CPh | 48% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
2.7.1.191 | additional information | the enzyme possesses a strict acceptor specificity toward Glc-beta1,3-GalNAc-diphospho-lipids | Clostridioides difficile | ? | - |
- |
|
2.7.1.191 | additional information | the enzyme shows no activity with GDP-Glc2dO, GDP-L-fucose, GDP-L-galactose, GTP, UDP-D-mannose, UDP-D-glucose, and CMPNeu5Ac | Clostridioides difficile | ? | - |
- |
|
2.7.1.191 | additional information | the enzyme possesses a strict acceptor specificity toward Glc-beta1,3-GalNAc-diphospho-lipids | Clostridioides difficile 630 | ? | - |
- |
|
2.7.1.191 | additional information | the enzyme shows no activity with GDP-Glc2dO, GDP-L-fucose, GDP-L-galactose, GTP, UDP-D-mannose, UDP-D-glucose, and CMPNeu5Ac | Clostridioides difficile 630 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.191 | ? | x * 50000, recombinant enzyme, SDS-PAGE | Clostridioides difficile |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.191 | CD2775 | - |
Clostridioides difficile |
2.7.1.191 | mannosyl-1-phosphotransferase | - |
Clostridioides difficile |
2.7.1.191 | ManPT | - |
Clostridioides difficile |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.191 | 2.248 | - |
GDP-D-mannose | at pH 8.0 and 37°C | Clostridioides difficile | |
2.7.1.191 | 2.738 | - |
Glcbeta1,3-GalNAc-PP-CPh | at pH 8.0 and 37°C | Clostridioides difficile |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.191 | metabolism | the enzyme is responsible for the formation of the Man-alpha1-P-6-Glc linkage in Clostridium difficile polysaccharide II | Clostridioides difficile |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.191 | 2.931 | - |
Glcbeta1,3-GalNAc-PP-CPh | at pH 8.0 and 37°C | Clostridioides difficile | |
2.7.1.191 | 3.903 | - |
GDP-D-mannose | at pH 8.0 and 37°C | Clostridioides difficile |