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Literature summary extracted from

  • Marathe, A.; Krishnan, V.; Mahajan, M.M.; Thimmegowda, V.; Dahuja, A.; Jolly, M.; Praveen, S.; Sachdev, A.
    Characterization and molecular modeling of inositol 1,3,4 tris phosphate 5/6 kinase-2 from Glycine max (L) Merr. comprehending its evolutionary conservancy at functional level (2018), 3 Biotech, 8, 50 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.159 single copy Itpk2 gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged GmITPK2 in Escherichia coli strain BL21(DE3) Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.159 cytosol cytosolic localization of GmITPK2 protein is predicted by CELLO tool with a reliability index of 2.976 Glycine max 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.159 Mg2+ required Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Glycine max
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Glycine max
-
 ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.159 Glycine max A7X665 cv. Pusa-16, grown in the fields of Division of Genetics, I.A.R.I, New Delhi
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.159 flower
-
 Glycine max
-
2.7.1.159 root
-
 Glycine max
-
2.7.1.159 seed spatio-temporal transcript profiling signified GmItpk2 to be seed-specific, with higher transcript levels in the early stage of seed development, GmItpk2 expression profiling overview Glycine max
-
2.7.1.159 seedling
-
 Glycine max
-
2.7.1.159 stem
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Glycine max ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Glycine max ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.159 ? x * 35000, SDS-PAGE Glycine max
2.7.1.159 More the predicted three-dimensional model confirms 12 alpha-helices and 14 beta-barrel sheets with ATP-binding site close to beta-sheet present towards the C-terminus of the protein molecule. Secondary structure prediction of GmITPK2 using PDBSum tool Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.7.1.159 GmItpk2
-
 Glycine max
2.7.1.159 inositol 1,3,4 tris phosphate 5/6 kinase-2
-
 Glycine max
2.7.1.159 inositol 1,3,4 trisphosphate kinase-2
-
 Glycine max
2.7.1.159 inositol 1,4,5 trisphosphate 3/6 kinase
-
 Glycine max
2.7.1.159 Ipk2
-
 Glycine max
2.7.1.159 ITPK2
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.159 ATP
-
 Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.159 Glycine max sequence calculation
-
 5.9

Expression

EC Number Organism Comment Expression
2.7.1.159 Glycine max GmITPK2 is an early dehydration responsive gene which is also induced by dehydration and exogenous treatment with abscisic acid up

General Information

EC Number General Information Comment Organism
2.7.1.159 evolution phylogenetic tree showing the evolutionary relatedness of GmITPK2 with ITPKs of monocot and dicot species using Entamoeba histolytica (EhITPK) as an outgroup member Glycine max
2.7.1.159 metabolism following the formation of myo-inositol-3-phosphate, sequential phosphorylation reactions via action of various inositol phosphate kinases-myo-inositol kinase (Mik), inositol 1,3,4-trisphosphate kinase (Itpk), inositol 1,4,5-trisphosphate 3/6 kinase (Ipk2), and inositol 1,3,4,5,6-pentakisphosphate kinase (Ipk1) lead to phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) synthesis via the lipid-independent pathway and fine-tune the phosphorous flux towards PA accumulation Glycine max
2.7.1.159 additional information enzyme homology-based modeling and structure comparisons, overview. Active site mapping and molecular docking with ATP Glycine max
2.7.1.159 physiological function inositol 1,3,4-trisphosphate kinase-2 (GmItpk2), catalyzing the ATP-dependent phosphorylation of inositol 1,3,4-trisphosphate (IP3) to inositol 1,3,4,5-tetraphosphate or inositol 1,3,4,6-tetraphosphate, is a key enzyme diverting the flux of inositol phosphate pool towards phytate biosynthesis Glycine max