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Literature summary extracted from

  • Liu, B.; Ni, J.; Shen, Y.
    Cloning, expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilic archaeon Pyrococcus horikoshii (2006), Wei Sheng Wu Xue Bao, 46, 255-258 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.136 amplified by polymerase chain reaction, cloned into expression vector pET15b, and expressed in E. coli BL21-codonPlus (DE3)-RIL Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.136 N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii chitin degradation pathway in hyperthermophilic archaea beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii ATCC 700860 chitin degradation pathway in hyperthermophilic archaea beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.136 Pyrococcus horikoshii O58235
-
-
3.5.1.136 Pyrococcus horikoshii ATCC 700860 O58235
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.136 N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O chitin degradation pathway in hyperthermophilic archaea Pyrococcus horikoshii beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii ATCC 700860 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
3.5.1.136 N,N'-diacetylchitobiose + H2O chitin degradation pathway in hyperthermophilic archaea Pyrococcus horikoshii ATCC 700860 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.136 ? x * 31600, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.5.1.136 diacetylchitobiose deacetylase
-
 Pyrococcus horikoshii
3.5.1.136 PH0499
-
 Pyrococcus horikoshii