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Literature summary extracted from

  • Lu, H.; Chanco, E.; Zhao, H.
    CmlI is an N-oxygenase in the biosynthesis of chloramphenicol (2012), Tetrahedron, 68, 7651-7654 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.67 expression in Escherichia coli Streptomyces venezuelae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.67 Iron non-heme di-iron enzyme, iron content is 1.2-1.4 mol per mol of enzyme Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2 Streptomyces venezuelae
-
 ?
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2 Streptomyces venezuelae DSM 40230
-
 ?
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2 Streptomyces venezuelae
-
 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2 Streptomyces venezuelae DSM 40230
-
 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.67 Streptomyces venezuelae F2RB83
-
-
1.14.99.67 Streptomyces venezuelae DSM 40230 F2RB83
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae ?
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae DSM 40230 ?
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae chloramphenicol + phenazine ethosulfate + 2 H2O
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae DSM 40230 chloramphenicol + phenazine ethosulfate + 2 H2O
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2
-
 Streptomyces venezuelae chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2
-
 Streptomyces venezuelae DSM 40230 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
1.14.99.67 additional information no acceptor: flavin mononucleotide. The reaction shows a biphasic behavior, with a fast step consuming significant amounts of substrate within 15 s, and a slower step afterward Streptomyces venezuelae ?
-
-
1.14.99.67 additional information no acceptor: flavin mononucleotide. The reaction shows a biphasic behavior, with a fast step consuming significant amounts of substrate within 15 s, and a slower step afterward Streptomyces venezuelae DSM 40230 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.14.99.67 cmlI
-
 Streptomyces venezuelae

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.67 additional information no acceptor: flavin mononucleotide Streptomyces venezuelae

General Information

EC Number General Information Comment Organism
1.14.99.67 physiological function CmlI is a non-heme di-iron enzyme catalyzing N-oxygenation of an amine group, one of the steps in the biosynthesis of the antibiotic chloramphenicol Streptomyces venezuelae