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Literature summary extracted from

  • Ortiz-Soto, M.E.; Reising, S.; Schlosser, A.; Seibel, J.
    Structural and functional role of disulphide bonds and substrate binding residues of the human beta-galactoside alpha-2,3-sialyltransferase 1 (hST3Gal1) (2019), Sci. Rep., 9, 17993 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.4 expressed in Escherichia coli Origami2 (DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.4 C59S/C64S the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 H299A inactive Homo sapiens
2.4.3.4 H316A inactive Homo sapiens
2.4.3.4 N147S the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 N170A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 S148A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 V315A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 V315Y the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 Y191A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 Y230A the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 Y230F the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens
2.4.3.4 Y300A inactive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.4 0.02
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme C59S/C64S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.026
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.096
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme N170A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.105
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme S148A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.106
-
 CMP-N-acetyl-beta-neuraminate wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.121
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme C59S/C64S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.176
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y230F, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.18
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme V315A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.184
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme N170A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.205
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y230A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.211
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme N147S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.271
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme V315Y, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.352
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y230F, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.444
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y191A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.481
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme S148A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.549
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme V315Y, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.668
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme N147S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.854
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y230A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.876
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y191A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 1.388
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme V315A, at pH 7.4 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.4 CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R Homo sapiens
-
 CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.4 Homo sapiens Q11201
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.4 His-Select nickel affinity column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.4 CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
 Homo sapiens CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Homo sapiens CMP + ?
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
-
 Homo sapiens CMP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.4 ? x * 66200, maltose-binding-protein-fused recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.3.4 beta-galactoside alpha-2,3-sialyltransferase 1
-
 Homo sapiens
2.4.3.4 ST3GAL1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.3.4 0.13
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme V315Y, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.2
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme V315Y, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.47
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y191A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.62
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme N170A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.63
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y191A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.65
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme N170A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.8
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme N147S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 1.05
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme V315A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 1.21
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme N147S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.25
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme C59S/C64S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.28
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme C59S/C64S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.32
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme S148A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.42
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme V315A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.5
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y230A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.7
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y230F, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 2.97
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y230A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.15
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.35
-
 CMP-N-acetyl-beta-neuraminate wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.47
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y230F, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.65
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme S148A, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.3.4 0.37
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme V315Y, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.47
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme V315Y, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 0.72
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y191A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 1.05
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y191A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 1.75
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme V315A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 1.87
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme N147S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.35
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme N170A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.47
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y230A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 3.78
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme N147S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 5.83
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme V315A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 6.77
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme N170A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 7.58
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme S148A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 9.85
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme Y230F, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 12.2
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y230A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 15.33
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme Y230F, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 18.6
-
 CMP-N-acetyl-beta-neuraminate mutant enzyme C59S/C64S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 22.07
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme S148A, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 31.6
-
 CMP-N-acetyl-beta-neuraminate wild type enzyme, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 114.17
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside mutant enzyme C59S/C64S, at pH 7.4 and 37°C Homo sapiens
2.4.3.4 121.15
-
 benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside wild type enzyme, at pH 7.4 and 37°C Homo sapiens