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Literature summary extracted from

  • Wangpaiboon, K.; Padungros, P.; Nakapong, S.; Charoenwongpaiboon, T.; Rejzek, M.; Field, R.A.; Pichyangkura, R.
    An alpha-1,6-and alpha-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties (2018), Sci. Rep., 8, 8340 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.140 additional information no effect by 10 mM EDTA Leuconostoc citreum
2.4.1.140 Triton X-100 21% activation at 0.1% v/v Leuconostoc citreum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.140 gene Lcalt, rcombinant expression in Escherichia coli strain BL21(DE3) Leuconostoc citreum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.140 Cu2+ 86% inhibition at 10 mM Leuconostoc citreum
2.4.1.140 Fe3+ 94% inhibition at 10 mM Leuconostoc citreum
2.4.1.140 additional information no effect by 10 mM EDTA Leuconostoc citreum
2.4.1.140 Zn2+ 39% inhibition at 10 mM Leuconostoc citreum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.140 32.2
-
 sucrose pH 5.0, 40°C, recombinant enzyme Leuconostoc citreum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.140 Ca2+ 22% activation at 10 mM Leuconostoc citreum
2.4.1.140 Co2+ 10% activation at 10 mM Leuconostoc citreum
2.4.1.140 Mn2+ activates 2.4fold, 138% Leuconostoc citreum
2.4.1.140 additional information no effect by 10 mM Mg2+ Leuconostoc citreum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.140 Leuconostoc citreum D3JC16
-
-
2.4.1.140 Leuconostoc citreum ABK-1 D3JC16
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.140 rcombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and dialysis Leuconostoc citreum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.140 34.7
-
 purified recombinant enzyme, pH 5.0, 40°C Leuconostoc citreum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.140 2 sucrose
-
 Leuconostoc citreum ?
-
 ?
2.4.1.140 2 sucrose
-
 Leuconostoc citreum ABK-1 ?
-
 ?
2.4.1.140 additional information product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering Leuconostoc citreum ?
-
-
2.4.1.140 additional information product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering Leuconostoc citreum ABK-1 ?
-
-
2.4.1.140 sucrose + alpha-D-glucan
-
 Leuconostoc citreum ?
-
 ?
2.4.1.140 sucrose + alpha-D-glucan
-
 Leuconostoc citreum ABK-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.140 alternansucrase
-
 Leuconostoc citreum
2.4.1.140 Lcalt
-
 Leuconostoc citreum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.140 40
-
 recombinant enzyme Leuconostoc citreum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.140 30 55 activity range, profile overview Leuconostoc citreum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.140 290
-
 sucrose pH 5.0, 40°C, recombinant enzyme Leuconostoc citreum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.140 5
-
 recombinant enzyme Leuconostoc citreum

General Information

EC Number General Information Comment Organism
2.4.1.140 evolution the enzyme belongs to the CAZy glycoside hydrolase family 70 (GH70) Leuconostoc citreum
2.4.1.140 physiological function alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as alternan. The insoluble polymer alternan has alternating alpha-1,6 and alpha-1,3 glycosidic linkages as has been judged by using 13C NMR spectroscopy and methylation analysis, partially hydrolysed product analysis, and enzymatic analysis Leuconostoc citreum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.140 9
-
 sucrose pH 5.0, 40°C, recombinant enzyme Leuconostoc citreum