Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rout, M.K.; Lee, B.L.; Lin, A.; Xiao, W.; Spyracopoulos, L.
    Active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K (2018), Sci. Rep., 8, 7002 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.23 gene UBE2K, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.23 D127G site-directed mutagenesis Homo sapiens
2.3.2.23 additional information molecular dynamics simulations for E2-25K and the Q126L/D127G active site gate mutants Homo sapiens
2.3.2.23 Q126A site-directed mutagenesis Homo sapiens
2.3.2.23 Q126G site-directed mutagenesis Homo sapiens
2.3.2.23 Q126I site-directed mutagenesis Homo sapiens
2.3.2.23 Q126L site-directed mutagenesis Homo sapiens
2.3.2.23 Q126V site-directed mutagenesis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.23 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.23 Homo sapiens P61086
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.23 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage by TEV protease, dialysis, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.23 additional information determination of the E1 catalyzed E2-25K-Ub thioester conjugation reaction Homo sapiens ?
-
-
2.3.2.23 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.23 E2-25K
-
 Homo sapiens
2.3.2.23 ubiquitination enzyme
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.23 20
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.23 8
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.2.23 additional information active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K. NMR study of purified recombinant detagged wild-type and mutant enzymes, analysis of enzyme structure and E2-25K-ubiquitin interaction, overview. Molecular dynamics simulations for E2-25K and the Q126L/D127G active site gate mutants Homo sapiens