Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kalita, J.; Shukla, R.; Shukla, H.; Gadhave, K.; Giri, R.; Tripathi, T.
    Comprehensive analysis of the catalytic and structural properties of a mu-class glutathione S-transferase from Fasciola gigantica (2017), Sci. Rep., 7, 17547 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Fasciola gigantica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 Cibacron blue
-
 Fasciola gigantica
2.5.1.18 additional information not inhibited by bromosulfophthalein Fasciola gigantica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.00958
-
 trans-2-nonenal at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 0.01424
-
 trans-4-phenyl-3-butene-2-one at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 0.04835
-
 hexa-2,4-dienal at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 0.04835
-
 4-nitroquinoline-1-oxide at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 0.2313
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Fasciola gigantica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 50000
-
 gel filtration Fasciola gigantica

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Fasciola gigantica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 GST affinity column chromatography Fasciola gigantica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 4.04
-
 with hexa-2,4-dienal as substrate, at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 4.54
-
 with trans-4-phenyl-3-butene-2-one as substrate, at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 5.1
-
 with trans-2-nonenal as substrate, at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 13.14
-
 with 4-nitroquinoline-1-oxide as substrate, at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 68.54
-
 with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.5 and 30°C Fasciola gigantica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Fasciola gigantica chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + 4-nitroquinoline-1-oxide
-
 Fasciola gigantica ?
-
 ?
2.5.1.18 glutathione + hexa-2,4-dienal
-
 Fasciola gigantica ?
-
 ?
2.5.1.18 glutathione + trans-2-nonenal
-
 Fasciola gigantica ?
-
 ?
2.5.1.18 glutathione + trans-4-phenyl-3-butene-2-one
-
 Fasciola gigantica ?
-
 ?
2.5.1.18 additional information no activity against 1,2-dichloro-4-nitrobenzene and ethacrynic acid Fasciola gigantica ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.18 homodimer 2 * 25000, SDS-PAGE Fasciola gigantica

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST1
-
 Fasciola gigantica
2.5.1.18 mu-class glutathione S-transferase
-
 Fasciola gigantica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 40
-
-
 Fasciola gigantica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 20 70 the enzyme activity is reduced to approximately 50% at 20°C, to 25% at 60°C and 20% at 70°C Fasciola gigantica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 3.47
-
 hexa-2,4-dienal at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 3.9
-
 4-nitroquinoline-1-oxide at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 4.38
-
 trans-2-nonenal at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 11.28
-
 trans-4-phenyl-3-butene-2-one at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 58.77
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Fasciola gigantica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5
-
-
 Fasciola gigantica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6 9 the enzyme is highly active between pH 6.0 and 9.0. At pH values below 6.0 and above 9.0, the activity decreases substantially Fasciola gigantica

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00135
-
 at pH 7.5 and 25°C Fasciola gigantica Cibacron blue

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 39.7
-
 hexa-2,4-dienal at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 233
-
 trans-4-phenyl-3-butene-2-one at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 254
-
 1-chloro-2,4-dinitrobenzene at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 274
-
 4-nitroquinoline-1-oxide at pH 7.5 and 30°C Fasciola gigantica
2.5.1.18 457
-
 trans-2-nonenal at pH 7.5 and 30°C Fasciola gigantica