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Literature summary extracted from

  • Xu, Z.; Ma, J.; Wang, F.; Ma, H.; Wang, Q.; Xiong, A.
    Identification and characterization of DcUCGalT1, a galactosyltransferase responsible for anthocyanin galactosylation in purple carrot (Daucus carota L.) taproots (2016), Sci. Rep., 6, 27356 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.294 expression in Escherichia coli Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.294 0.041
-
 pelargonidin pH 8.6, 30°C Daucus carota
2.4.1.294 0.066
-
 cyanidin pH 8.6, 30°C Daucus carota
2.4.1.294 0.14
-
 peonidin pH 8.6, 30°C Daucus carota
2.4.1.294 0.398
-
 UDP-alpha-D-galactose cosubstrate cyanidin, pH 8.6, 30°C Daucus carota
2.4.1.294 0.75
-
 UDP-alpha-D-galactose cosubstrate pelargonidin, pH 8.6, 30°C Daucus carota
2.4.1.294 1.482
-
 UDP-alpha-D-galactose cosubstrate peonidin, pH 8.6, 30°C Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.294 Daucus carota A0A0G2UEA5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.294 root taproot Daucus carota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.294 additional information no donor substrates: UDP-glucose, UDP-xylose Daucus carota ?
-
-
2.4.1.294 UDP-alpha-D-galactose + cyanidin
-
 Daucus carota UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + kaempferol
-
 Daucus carota UDP + kaempferol 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + pelargonidin
-
 Daucus carota UDP + pelargonidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + peonidin
-
 Daucus carota UDP + peonidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + quercetin
-
 Daucus carota UDP + quercetin 3-O-beta-D-galactoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.294 ? x * 49370, calculated from sequence, x * 55000, SDS-PAGE of recombinant protein including tags Daucus carota

Synonyms

EC Number Synonyms Comment Organism
2.4.1.294 UCGalT1
-
 Daucus carota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.294 30
-
-
 Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.294 8.6
-
-
 Daucus carota

Expression

EC Number Organism Comment Expression
2.4.1.294 Daucus carota the expression level of UCGalT1 is positively correlated with anthocyanin biosynthesis in carrots additional information