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Literature summary extracted from

  • Singh, G.; Tiwari, M.; Singh, S.P.; Singh, S.; Trivedi, P.K.; Misra, P.
    Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera (2016), Sci. Rep., 6, 25562 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.173 additional information analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifer. Phenotype, overview Withania somnifera
2.4.1.173 additional information analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifera. Phenotype, overview Withania somnifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.173 UDP-glucose + a sterol Withania somnifera
-
 UDP + a sterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + beta-sitosterol Withania somnifera
-
 UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol Withania somnifera
-
 UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + withaferin A Withania somnifera
-
 UDP + ?
-
 ?
2.4.1.173 UDP-glucose + withanolide A Withania somnifera
-
 UDP + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Withania somnifera
-
-
-
2.4.1.173 Withania somnifera Q2I014
-
-
2.4.1.173 Withania somnifera Q2I015
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 root
-
 Withania somnifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 UDP-glucose + a sterol
-
 Withania somnifera UDP + a sterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + beta-sitosterol
-
 Withania somnifera UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol
-
 Withania somnifera UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + withaferin A
-
 Withania somnifera UDP + ?
-
 ?
2.4.1.173 UDP-glucose + withanolide A
-
 Withania somnifera UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.173 SGTL1
-
 Withania somnifera
2.4.1.173 SgtL2
-
 Withania somnifera
2.4.1.173 SgtL4
-
 Withania somnifera
2.4.1.173 sterol glycosyltransferase
-
 Withania somnifera

General Information

EC Number General Information Comment Organism
2.4.1.173 malfunction analysis of the function of SGTs by silencing SGTL1, SGTL2 and SGTL4 in Withania somnifera. Downregulation of SGTs by artificial miRNAs leads to the enhanced accumulation of withanolide A, withaferin A, sitosterol, stigmasterol and decreased content of withanoside V in virus induced gene silencing (VIGS) lines. This is further correlated with increased expression of WsHMGR, WsDXR, WsFPPS, WsCYP710A1, WsSTE1 and WsDWF5 genes, involved in withanolide biosynthesis. These variations of withanolide concentrations in silenced lines result in pathogen susceptibility as compared to control plants. The infection of Alternaria alternata causes increased salicylic acid, callose deposition, superoxide dismutase and H2O2 in aMIR-VIGS lines. The expression of biotic stress related genes, namely, WsPR1, WsDFS, WsSPI and WsPR10 is also enhanced in aMIR-VIGS lines in time dependent manner. Salicylic acid level increases the expression of defence related genes in silenced lines. A positive feedback regulation of withanolide biosynthesis occurs by silencing of SGTLs which results in reduced biotic tolerance Withania somnifera
2.4.1.173 physiological function sterol glycosyltransferases (SGTs) catalyse transfer of glycon moiety to sterols and their related compounds to produce diverse glyco-conjugates or steryl glycosides with different biological and pharmacological activities. SGTs from Withania somnifera play a role in abiotic stresses Withania somnifera