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Literature summary extracted from

  • Ma, L.; Li, Y.; Meng, L.; Deng, H.; Li, Y.; Zhang, Q.; Diao, A.
    Biological fluorination from the sea Discovery of a SAM-dependent nucleophilic fluorinating enzyme from the marine-derived bacterium Streptomyces xinghaiensis NRRL B24674 (2016), RSC Adv., 6, 27047-27051 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.63 D16E complete loss of activity, residue is crucial for substrate SAM binding Streptomyces xinghaiensis
2.5.1.63 F156A complete loss of activity, residue provides the rear of the hydrophobic pocket Streptomyces xinghaiensis
2.5.1.63 S158A 20% of wild-type activity Streptomyces xinghaiensis
2.5.1.63 T80A complete loss of activity Streptomyces xinghaiensis
2.5.1.63 T80S 40% loss of activity Streptomyces xinghaiensis
2.5.1.63 Y77A almost complete loss of activity Streptomyces xinghaiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.63 Ca2+ about 25% loss of activity Streptomyces xinghaiensis
2.5.1.63 Cu2+ about 70% loss of activity Streptomyces xinghaiensis
2.5.1.63 EDTA about 40% loss of activity Streptomyces xinghaiensis
2.5.1.63 Fe2+ about 30% loss of activity Streptomyces xinghaiensis
2.5.1.63 Mg2+ about 40% loss of activity Streptomyces xinghaiensis
2.5.1.63 Mn2+ about 35% loss of activity Streptomyces xinghaiensis
2.5.1.63 Ni2+ about 30% loss of activity Streptomyces xinghaiensis
2.5.1.63 Sn2+ about 30% loss of activity Streptomyces xinghaiensis
2.5.1.63 Zn2+ about 50% loss of activity Streptomyces xinghaiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.007
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis
2.5.1.63 8.2
-
 fluoride pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces xinghaiensis A0A068VNW5
-
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.63 25°C, 50 mM Tris-HCl, pH 7.0, stable for 4 days Streptomyces xinghaiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + chloride
-
 Streptomyces xinghaiensis 5'-deoxy-5'-chloroadenosine + L-methionine
-
 r
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces xinghaiensis 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 40
-
-
 Streptomyces xinghaiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.63 0.0046
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.63 4 5
-
 Streptomyces xinghaiensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.63 0.658
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces xinghaiensis