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Literature summary extracted from

  • Jackson, E.K.; Gillespie, D.G.; Cheng, D.; Mi, Z.; Menshikova, E.V.
    Characterization of the N6-etheno-bridge method to assess extracellular metabolism of adenine nucleotides detection of a possible role for purine nucleoside phosphorylase in adenosine metabolism (2020), Purinergic Signal., 16, 187-211 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 forodesine
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.1 additional information Rattus norvegicus in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine ?
-
-
2.4.2.1 additional information Rattus norvegicus Wistar-Kyoto in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Homo sapiens P00491
-
-
2.4.2.1 Rattus norvegicus P85973
-
-
2.4.2.1 Rattus norvegicus Wistar-Kyoto P85973
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 commercial preparation recombinant enzyme Homo sapiens
-
2.4.2.1 fibroblast
-
 Rattus norvegicus
-
2.4.2.1 heart
-
 Rattus norvegicus
-
2.4.2.1 kidney
-
 Rattus norvegicus
-
2.4.2.1 mesangial cell
-
 Rattus norvegicus
-
2.4.2.1 vascular smooth muscle cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 7-methyl-6-thioguanosine + phosphate
-
 Homo sapiens 7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate complete conversion Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 additional information in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine Rattus norvegicus ?
-
-
2.4.2.1 additional information the recombinant enzyme does not metabolize adenosine to adenine or N6-etheno-adenosine to N6-etheno-adenine Homo sapiens ?
-
-
2.4.2.1 additional information in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine Rattus norvegicus Wistar-Kyoto ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.2.1 monomer the commercial recombinant enzyme is in monomeric form Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNPase
-
 Homo sapiens
2.4.2.1 PNPase
-
 Rattus norvegicus
2.4.2.1 purine nucleoside phosphorylase
-
 Homo sapiens
2.4.2.1 purine nucleoside phosphorylase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 30
-
 assay at Homo sapiens
2.4.2.1 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.4
-
 assay at Homo sapiens
2.4.2.1 7.4
-
 assay at Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.4.2.1 additional information N6-etheno-ATP and its downstream metabolites are devoid of pharmacological activity Rattus norvegicus
2.4.2.1 physiological function PNP catalyzes a reversible phosphorolysis of the N-glycosidic bond in natural purine nucleosides, as well as inmany purine analogues, some of them displaying marked biological and pharmacological activity Homo sapiens
2.4.2.1 physiological function PNP catalyzes a reversible phosphorolysis of the N-glycosidic bond in natural purine nucleosides, as well as inmany purine analogues, some of them displaying marked biological and pharmacological activity Rattus norvegicus