Literature summary extracted from

Chaurasia, N.; Mishra, Y.; Chatterjee, A.; Rai, R.; Yadav, S.; Rai, L.C.
Overexpression of phytochelatin synthase (pcs) enhances abiotic stress tolerance by altering the proteome of transformed Anabaena sp. PCC 7120 (2017), Protoplasma, 254, 1715-1724 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.2.15	gene alr0975 or pcs, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha, and functional constitutive overexpression in Anabaena sp. strain PCC 7120, semiquantitative RT-PCR enzyme expression analysis	Nostoc sp. PCC 7120 = FACHB-418

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.3.2.15	additional information	an extra copy of phytochelatin-encoding gene phytochelatin synthase (pcs) is constitutively expressed in Anabaena sp. strain PCC7120 (An7120), an integrative expression vector pFPN is used for incorporating a cassette of genes for genomic integration and expression. Comparative growth behavior of wild-type strain and overexpressed strain AnFPN-pcs under LC50 doses of UV-B, salt, heat, copper, carbofuran, and cadmium resulted in decrement in specific growth rate by 26, 21, 27, 13, 30, and 17%, respectively, in An7120 compared to AnFPN-pcs thereby suggesting that the transformed cyanobacterium AnFPN-pcs has developed tolerance and hence shows better growth performances under different abiotic stresses. Comparison of differentially expressed proteins in An7120 and AnFPN-pcs, and dynamics of differentially expressed proteins in An7120 and AnFPN-pcs, overview	Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.2.15	glutathione + [Glu(-Cys)]n-Gly	Nostoc sp. PCC 7120 = FACHB-418	-	Gly + [Glu(-Cys)]n+1-Gly	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.2.15	Nostoc sp. PCC 7120 = FACHB-418	Q8YY76	SAG 25.82, UTEX 2576, i.e. Anabaena sp. PCC 7120	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.2.15	glutathione + [Glu(-Cys)]n-Gly	-	Nostoc sp. PCC 7120 = FACHB-418	Gly + [Glu(-Cys)]n+1-Gly	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.3.2.15	alr0975	-	Nostoc sp. PCC 7120 = FACHB-418
2.3.2.15	PCS	-	Nostoc sp. PCC 7120 = FACHB-418
2.3.2.15	phytochelatin synthase	-	Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.2.15	37	-	assay at	Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.2.15	8	-	assay at	Nostoc sp. PCC 7120 = FACHB-418

General Information

EC Number	General Information	Comment	Organism
2.3.2.15	physiological function	role of of enzyme PCS in abiotic stress tolerance	Nostoc sp. PCC 7120 = FACHB-418

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Release 2023.1 (January 2023)