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Literature summary extracted from

  • Yao, J.; Subramanian, C.; Rock, C.O.; Jackowski, S.
    Human pantothenate kinase 4 is a pseudo-pantothenate kinase (2019), Protein Sci., 28, 1031-1047 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 gene PANK3, sequence comparisons and phylogenetic analysis Homo sapiens
2.7.1.33 gene PANK4, sequence comparisons and phylogenetic analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.33 E138V site-directed mutagenesis, inactive mutant Homo sapiens
2.7.1.33 E138V naturally occuring mutation in isozyme PanK4, inactive mutant Homo sapiens
2.7.1.33 additional information human PANK4, encode Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity Homo sapiens
2.7.1.33 R207W site-directed mutagenesis, inactive mutant Homo sapiens
2.7.1.33 R207W naturally occuring mutation in isozyme PanK4, inactive mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 acetyl-CoA
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 additional information
-
 additional information kinetics Homo sapiens
2.7.1.33 0.0031
-
 ATP pH 7.5, 37°C, wild-type enzyme Homo sapiens
2.7.1.33 0.017
-
 (R)-pantothenate pH 7.5, 37°C, wild-type enzyme Homo sapiens
2.7.1.33 0.025
-
 ATP pH 7.5, 37°C, mutant PanK3 R107W Homo sapiens
2.7.1.33 0.833
-
 (R)-pantothenate pH 7.5, 37°C, mutant PanK3 R107W Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Homo sapiens
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Homo sapiens
-
-
-
2.7.1.33 Homo sapiens Q9H999
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Homo sapiens ADP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 HsPANK3
-
 Homo sapiens
2.7.1.33 HsPANK4
-
 Homo sapiens
2.7.1.33 PanK3
-
 Homo sapiens
2.7.1.33 PanK4
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.33 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.33 evolution the human genome encodes three well-characterized and nearly identical pantothenate kinases (PANK1-3) plus a putative bifunctional protein (PANK4) with a predicted amino-terminal pantothenate kinase domain fused to a carboxy-terminal phosphatase domain. Structural and phylogenetic analyses show that all active, characterized PANKs contain the key catalytic residues Glu138 and Arg207 (HsPANK3 numbering). All amniote PANK4s, including human PANK4, encode Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity. Human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi. Evolutionary history of PANK, phylogenetic analysis, overview Homo sapiens
2.7.1.33 evolution the human genome encodes three well-characterized and nearly identical pantothenate kinases (PANK1-3) plus a putative bifunctional protein (PANK4) with a predicted N-terminal pantothenate kinase domain fused to a C-terminal phosphatase domain. Structural and phylogenetic analyses show that all active, characterized PANKs contain the key catalytic residues Glu138 and Arg207 (HsPANK3 numbering). All amniote PANK4s, including human PANK4, encode Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity. Human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi. Evolutionary history of PANK, overview Homo sapiens
2.7.1.33 malfunction human PANK3 is inactivated by mutations Glu138Val and Arg207Trp Homo sapiens
2.7.1.33 malfunction human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi. Pank4 encodes Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity Homo sapiens
2.7.1.33 additional information sequence comparison of isozyme PanK3 and PanK4 Homo sapiens
2.7.1.33 physiological function pantothenate kinase generates 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) biosynthesis. Critical roles of Glu138 and Arg207 in HsPANK3 Homo sapiens
2.7.1.33 physiological function pantothenate kinase generates 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) biosynthesis. Isozyme PanK4 is a putative bifunctional protein with a predicted amino-terminal pantothenate kinase domain fused to a carboxy-terminal phosphatase domain. HsPANK4 has reduced kinase activity prior to the catalytic residue substitutions in amniotes. Human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi Homo sapiens