Literature summary extracted from

Hiriart, Y.; Rossi, A.H.; Biedma, M.E.; Errea, A.J.; Moreno, G.; Cayet, D.; Rinaldi, J.; Blanca, B.; Sirard, J.C.; Goldbaum, F.; Berguer, P.; Rumbo, M.
Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella (2017), Protein Sci., 26, 1049-1059 .

Application

EC Number	Application	Comment	Organism
2.5.1.78	medicine	generation of a chimeric protein (BLS-FliC131) by fusing flagellin from Salmonella typhimurium in the N-termini of BLS. The fusion protein is recognized by anti-flagellin and anti-BLS antibodies, keeps the oligomerization capacity of BLS, without affecting the folding of the monomeric protein components. The thermal stability of each fusion partner is conserved. BLS-FliC131 retains the capacity of triggering TLR5. The humoral response against BLS elicited by BLS-FliC131 is stronger than the one elicited by equimolar amounts of BLS1FliC	Brucella abortus

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.5.1.78	additional information	generation of a chimeric protein (BLS-FliC131) by fusing flagellin from Salmonella typhimurium in the N-termini of BLS. The fusion protein is recognized by anti-flagellin and anti-BLS antibodies, keeps the oligomerization capacity of BLS, without affecting the folding of the monomeric protein components. The thermal stability of each fusion partner is conserved. BLS-FliC131 retains the capacity of triggering TLR5. The humoral response against BLS elicited by BLS-FliC131 is stronger than the one elicited by equimolar amounts of BLS1FliC	Brucella abortus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.5.1.78	Brucella abortus	Q2YKV1	-	-
2.5.1.78	Brucella abortus 2308	Q2YKV1	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
2.5.1.78	BLS	-	Brucella abortus
2.5.1.78	RibH2	-	Brucella abortus

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Release 2023.1 (January 2023)