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Literature summary extracted from

  • Sooklal, S.; De Koning, C.; Brady, D.; Rumbold, K.
    Identification and characterisation of a fluorinase from Actinopolyspora mzabensis (2020), Protein Expr. Purif., 166, 105508 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.63 expression in Escherichichia coli Actinopolyspora mzabensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.63 Cu2+ 1 mM, about 31% residual activity Actinopolyspora mzabensis
2.5.1.63 EDTA 1 mM, about 9% loss of activity Actinopolyspora mzabensis
2.5.1.63 additional information not inhibitory: Mg2+, Mn2+, Co2+ and Fe2+ Actinopolyspora mzabensis
2.5.1.63 Zn2+ 1 mM, about 31% residual activity Actinopolyspora mzabensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.63 Mg2+ 1 mM, 9.5% stimulation Actinopolyspora mzabensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Actinopolyspora mzabensis A0A1G9FQX8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.63 recovery from inclusion bodies after heterologous expression in Escherichia coli Actinopolyspora mzabensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.63 0.44
-
 pH 7.2, 65°C Actinopolyspora mzabensis

Storage Stability

EC Number Storage Stability Organism
2.5.1.63 25°C, 2 months, 26% residual activity Actinopolyspora mzabensis
2.5.1.63 4°C, 2 months, 84% residual activity Actinopolyspora mzabensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Actinopolyspora mzabensis 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 SAMN04487820_1174
-
 Actinopolyspora mzabensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 65
-
-
 Actinopolyspora mzabensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.63 80
-
 more than 80% of maximum activity Actinopolyspora mzabensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.63 7.2
-
-
 Actinopolyspora mzabensis