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Literature summary extracted from

  • Jeong, S.Y.; Jin, H.; Chang, J.H.
    Crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe (2019), PLoS ONE, 14, e0221975 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.1 expressed in Escherichia coli BL21(DE3) Star cells Schizosaccharomyces pombe

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.1 hanging drop vapor diffusion method, using 20% (w/v) polyethyleneglycol 8000 and 0.1 M sodium HEPES (pH 8.5) Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Schizosaccharomyces pombe
-
 oxaloacetate + L-glutamate
-
 r
2.6.1.1 L-aspartate + 2-oxoglutarate Schizosaccharomyces pombe 972
-
 oxaloacetate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Schizosaccharomyces pombe O42652
-
-
2.6.1.1 Schizosaccharomyces pombe 972 O42652
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 Ni-NTA column chromatography and Sephacryl S300 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Schizosaccharomyces pombe oxaloacetate + L-glutamate
-
 r
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Schizosaccharomyces pombe 972 oxaloacetate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.1 homodimer x-ray crystallography Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 AST
-
 Schizosaccharomyces pombe
2.6.1.1 L-aspartate aminotransferase
-
 Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate
-
 Schizosaccharomyces pombe