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Literature summary extracted from

  • Miller, B.R.; Kung, Y.
    Structural insight into substrate and product binding in an archaeal mevalonate kinase (2018), PLoS ONE, 13, e0208419 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B Methanosarcina mazei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.36 purified enzyme in mevalonate-bound and 5-phosphomevalonate-bound form, hanging drop vapor diffusion method, mixing of 11 mg/ml protein in 20 mM Tris pH 7.7, 10 mM MgCl2, and 10% glycerol, with 100 mM Bis-Tris pH 5.5, 200 mM sodium potassium tartrate, and 15-25% PEG 8000 or 10000, microseeding. Seeded drops contain 0.001 ml of protein with 1 mM (R)-mevalonate, 5 mM AMPPNP, and 5 mM MgCl2, and 800 nl crystallization solution, and 200 nl of the seed stock. Diffraction-quality plate crystals grow in crystallization solutions that contain 100 mM Bis-Tris, pH 5.5, 200-300 mM sodium potassium tartrate, and 15-25% PEG 8000 or 10000, X-ray diffraction structure determination and analysis at 2.1-2.46 A resolution, molecular replacement using the structure of the apo-MmMK from Methanosarcina mazei (PDB ID 4HAC) as the search model Methanosarcina mazei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 additional information
-
 additional information Michaelis-Menten kinetics Methanosarcina mazei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ required Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Methanosarcina mazei
-
 ADP + (R)-5-phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Methanosarcina mazei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Methanosarcina mazei ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 additional information analysis of substrate and product binding by archaeal mevalonate kinase compared to eukaryotic enzymes, a striking lack of direct interactions between this archaeal MK and its substrate is detected, substrate-binding site structure, overview Methanosarcina mazei ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 MmMK
-
 Methanosarcina mazei

General Information

EC Number General Information Comment Organism
2.7.1.36 metabolism mevalonate kinase (MK) is a key enzyme of the mevalonate pathway, which produces the biosynthetic precursors for steroids, including cholesterol, and isoprenoids, the largest class of natural products Methanosarcina mazei
2.7.1.36 additional information structure comparisons of MVKs from different species, active site structure and structure-function analysis, detailed overview Methanosarcina mazei