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Literature summary extracted from

  • Kajla, S.; Mukhopadhyay, A.; Pradhan, A.
    Development of transgenic Brassica juncea lines for reduced seed sinapine content by perturbing phenylpropanoid pathway genes (2017), PLoS ONE, 12, e0182747 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.120 gene SGT, recombinant expression of full-length enzyme in Brassica juncea via Agrobacterium tumefaciens mediated transformation Brassica juncea

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.120 additional information two genes are isolated from Brassica juncea and eight silencing constructs are developed using three different RNA silencing approaches viz. antisense RNA, RNAi and artificial microRNA. Transgenics in Brassica juncea are developed following Agrobacterium-mediated transformation. From a total of 1232 independent T0 transgenic events obtained using eight silencing constructs, 25 homozygous lines showing single gene inheritance are identified in the T2 generation. Reduction of seed sinapine content in these lines range from 15.8% to 67.2% compared to wild-type. Development of suppression constructs for SGT, development of silencing constructs for SGT genes viz. antisense RNA (BjSGTAS for SGT), RNAi (BjSGTRNAi for SGT), and artificial microRNA (BjSGTamiR38 and BjSGTamiR40 for SGT), genetic analysis of T2 transgenics and establishment of homozygous lines Brassica juncea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate Brassica juncea
-
 UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.120 Brassica juncea A0A0M3PZA4 cv. Varuna
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 seed expression analysis of SGT gene measured at different stages of seed development Brassica juncea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate
-
 Brassica juncea UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.120 BjSGT
-
 Brassica juncea
2.4.1.120 SGT
-
 Brassica juncea
2.4.1.120 SGT enzyme
-
 Brassica juncea
2.4.1.120 UDP-glucose: sinapate glucosyltransferase
-
 Brassica juncea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.120 30
-
 assay at Brassica juncea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.120 7
-
 assay at Brassica juncea

General Information

EC Number General Information Comment Organism
2.4.1.120 metabolism SGT and SCT, encoding enzymes UDP-glucose: sinapate glucosyltransferase and sinapoylglucose: choline sinapoyltransferase, respectively, are involved in the final two steps of sinapine biosynthetic pathway. Comparison of the two enzymes, overview Brassica juncea
2.4.1.120 additional information in-silico structural analysis and molecular docking. Three dimensional structure and binding mode of sinapate with SGT enzyme, overview Brassica juncea