EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.48 | drug development | RX-3117, an orally available cytidine analogue, is activated by UCK2 which may be used to select patients potentially sensitive to RX-3117 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.48 | additional information | enzyme silencing by transfection of A549 and SW1573 cell lines with UCK-siRNAs. Transfection of UCK1-siRNA efficiently downregulates UCK1-mRNA, but not UCK2-mRNA expression, and does not affect sensitivity to RX-3117. Transfection of UCK2-siRNA completely downregulates UCK2-mRNA and protein and protects both A549 and SW1573 against RX-3117 | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.48 | Mg2+ | required | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.48 | ATP + cytidine | Homo sapiens | - |
ADP + CMP | - |
? | |
2.7.1.48 | ATP + uridine | Homo sapiens | - |
ADP + UMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.48 | Homo sapiens | Q9BZX2 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.48 | A-549 cell | - |
Homo sapiens | - |
2.7.1.48 | additional information | enzyme activity in two panels of tumor cell lines and xenograft cells correlated only with UCK2-mRNA expression, but not with UCK1-mRNA. Moreover, accumulation of RX-3117 nucleotides correlates with UCK2 expression | Homo sapiens | - |
2.7.1.48 | SW-1573 cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.48 | ATP + 4-amino-1-[(1S,4R,5S)-2-fluoro-4,5-dihydroxy-3-(hydroxymethyl)cyclopent-2-en-1-yl]pyrimidin-2(1H)-one | - |
Homo sapiens | ADP + [(3S,4S,5R)-3-(4-amino-2-oxopyrimidin-1(2H)-yl)-2-fluoro-4,5-dihydroxycyclopent-1-en-1-yl]methyl phosphate | - |
ir | |
2.7.1.48 | ATP + cytidine | - |
Homo sapiens | ADP + CMP | - |
? | |
2.7.1.48 | ATP + fluorocyclopentenylcytosine | an orally available cytidine analogue, RX-3117 | Homo sapiens | ? | - |
? | |
2.7.1.48 | ATP + uridine | - |
Homo sapiens | ADP + UMP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.48 | uridine-cytidine kinase 2 | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.48 | physiological function | enzyme activity in two panels of tumor cell lines and xenograft cells correlated only with UCK2-mRNA expression, but not with UCK1-mRNA. Moreover, accumulation of RX-3117 nucleotides correlates with UCK2 expression | Homo sapiens |