Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Torini, J.R.; Brandao-Neto, J.; DeMarco, R.; Pereira, H.D.
    Crystal structure of Schistosoma mansoni adenosine phosphorylase/5'-methylthioadenosine phosphorylase and its importance on adenosine salvage pathway (2016), PLoS Negl. Trop. Dis., 10, e0005178 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 expressed in Escherichia coli BL21(DE3) cells Schistosoma mansoni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.28 in complex with adenine, 5'-deoxy-5'-methylthioadenosine, tubercidin or sulfate, hanging drop vapor diffusion method, using 100 mM Bis-Tris or MES buffer, pH 6.1-6.7 and 14-18% (w/v) PEG 3350 Schistosoma mansoni

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.28 N87T the mutant shows reduced catalytic efficiency compared to the wild type enzyme Schistosoma mansoni
2.4.2.28 Q289L the mutant shows reduced catalytic efficiency compared to the wild type enzyme Schistosoma mansoni
2.4.2.28 S12T the mutant shows reduced catalytic efficiency compared to the wild type enzyme Schistosoma mansoni
2.4.2.28 S12T/N87T the mutant shows increased catalytic efficiencies with 5'-deoxy-5'-methylthioadenosine and 2'-deoxyadenosine and decreased catalytic efficiency with adenosine compared to the wild type enzyme Schistosoma mansoni
2.4.2.28 S12T/N87T/Q289L the mutant shows reduced catalytic efficiency compared to the wild type enzyme Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.00071
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00099
-
 2'-deoxyadenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00122
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00181
-
 adenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.0021
-
 adenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.002124
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00249
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00302
-
 2'-deoxyadenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00312
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00314
-
 adenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.0033
-
 adenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00363
-
 5'-deoxy-5'-methylthioadenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00368
-
 adenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00389
-
 2'-deoxyadenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00397
-
 2'-deoxyadenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00652
-
 2'-deoxyadenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.00735
-
 adenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 0.01033
-
 2'-deoxyadenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.28 5'-deoxy-5'-methylthioadenosine + phosphate Schistosoma mansoni
-
 adenine + 5'-deoxy-5'-methyl-thio-D-ribose-1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate Schistosoma mansoni the enzyme can use adenosine with the same efficiency as 5'-deoxy-5'-methylthioadenosine adenine + D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Schistosoma mansoni I0B503
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28 Co-NTA agarose column chromatography Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 2'-deoxyadenosine + phosphate the enzyme can use adenosine with the same efficiency as 5'-deoxy-5'-methylthioadenosine Schistosoma mansoni adenine + 2'-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-deoxy-5'-methylthioadenosine + phosphate
-
 Schistosoma mansoni adenine + 5'-deoxy-5'-methyl-thio-D-ribose-1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate the enzyme can use adenosine with the same efficiency as 5'-deoxy-5'-methylthioadenosine Schistosoma mansoni adenine + D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 ? x * 33900, SDS-PAGE Schistosoma mansoni
2.4.2.28 ? x * 32914, calculated from amino acid sequence Schistosoma mansoni

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 5'-deoxy-5'-methylthioadenosine phosphorylase
-
 Schistosoma mansoni
2.4.2.28 5'-methylthioadenosine phosphorylase
-
 Schistosoma mansoni
2.4.2.28 MTAP
-
 Schistosoma mansoni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.28 48.44
-
 2'-deoxyadenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 62.63
-
 adenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 80.03
-
 2'-deoxyadenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 84.62
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 95.79
-
 adenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 98.42
-
 adenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 145.1
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 224.3
-
 2'-deoxyadenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 241.6
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 253.9
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 280.3
-
 adenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 299.2
-
 2'-deoxyadenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 308.9
-
 adenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 334.1
-
 adenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 372.8
-
 2'-deoxyadenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 416.2
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 441.2
-
 2'-deoxyadenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 443.8
-
 5'-deoxy-5'-methylthioadenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni

General Information

EC Number General Information Comment Organism
2.4.2.28 metabolism the enzyme is part of the purine pathway salvage Schistosoma mansoni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.28 18.97
-
 adenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 26.5
-
 2'-deoxyadenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 38.13
-
 adenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 42.71
-
 2'-deoxyadenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 45.88
-
 2'-deoxyadenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 46.86
-
 adenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 48.92
-
 2'-deoxyadenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 54.58
-
 adenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 56.49
-
 2'-deoxyadenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 81.37
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T/N87T/Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 83.94
-
 adenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 95.83
-
 2'-deoxyadenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 97.02
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme Q289L, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 106.4
-
 adenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 118.93
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 119.18
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme N87T, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 122.25
-
 5'-deoxy-5'-methylthioadenosine wild type enzyme, at pH 7.4 and 25°C Schistosoma mansoni
2.4.2.28 196.32
-
 5'-deoxy-5'-methylthioadenosine mutant enzyme S12T/N87T, at pH 7.4 and 25°C Schistosoma mansoni