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Literature summary extracted from

  • Veiseth, S.V.; Rahman, M.A.; Yap, K.L.; Fischer, A.; Egge-Jacobsen, W.; Reuter, G.; Zhou, M.M.; Aalen, R.B.; Thorstensen, T.
    The SUVR4 histone lysine methyltransferase binds ubiquitin and converts H3K9me1 to H3K9me3 on transposon chromatin in Arabidopsis (2011), PLoS Genet., 7, e1001325 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.B130 ubiquitin addition of free ubiquitin stimulates enzymatic activity of the SUVR4 protein on histone H3 but does not affect its specificity. In presence of ubiquitin, methylation of H3K9me1 modified peptides increases 2.5-3fold. Trimethylation of the H3K9me2 peptide is observed in the presence of ubiquitin Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.B130 additional information presence of trimethyl-H3K4 reduces SUVR4 activity significantly Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.B130 Arabidopsis thaliana Q8W595
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.B130 additional information in the absence of ubiquitin, SUVR4 converts 40.9% of the H3K9me1 peptide to H3K9me2, while 0% is converted to H3K9me3. In the presence of ubiquitin, 90.2% of the H3K9me1 peptide is converted to H3K9me2 while 3.5% is converted to H3K9me3. When H3K9me2 peptides are used as substrate, there is no conversion to H3K9me3 above background level in the absence of ubiquitin. When ubiquitin is present together with SUVR4, a 16.4% conversion from H3K9me2 to H3K9me3 is found Arabidopsis thaliana ?
-
-
2.1.1.B130 N6,N6-dimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-methionine
-
 Arabidopsis thaliana N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteine
-
 ?
2.1.1.B130 N6-methyl-L-lysyl9-[histone H3] + 2 S-adenosyl-L-methionine
-
 Arabidopsis thaliana N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + 2 S-adenosyl-L-homocysteine
-
 ?
2.1.1.B130 N6-methyl-L-lysyl9-[histone H3] + S-adenosyl-L-methionine
-
 Arabidopsis thaliana N6,N6-dimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.B130 SUVR4
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.1.1.B130 physiological function the N-terminal WIYLD domain of the SUVR4 HKMTase binds ubiquitin and the SUVR4 product specificity shifts from di- to trimethylation in the presence of free ubiquitin, enabling conversion of H3K9me1 to H3K9me3 in vitro. SUVR4 in vivo specifically converts H3K9me1 to H3K9me3 at transposons and pseudogenes and has a locus-specific repressive effect on the expression of such elements. This repression involves both DNA methylation-dependent and -independent mechanisms Arabidopsis thaliana