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Literature summary extracted from

  • Mukherjee, S.; Sengupta, S.; Mukherjee, A.; Basak, P.; Majumder, A.L.
    Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice (2019), Planta, 249, 891-912 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.123 gene GolS1, located on chromosome 3, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group
2.4.1.123 gene GolS2, located on chromosome 7, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-alpha-D-galactose + myo-inositol Oryza sativa Japonica Group
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 UDP + O-alpha-D-galactosyl-(1->3)-1D-myo-inositol
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.123 Oryza sativa Japonica Group A0A5S6R7K3
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2.4.1.123 Oryza sativa Japonica Group Q40710
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.123 recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.123 leaf in 15 DAG seedlings, mature full-length OsGolS1 is expressed only in leaf sheath of etiolated plants. In contrast, the q-PCR indicative of pre-mRNA accumulation relative to actin (housekeeping gene) expression shows a strikingly different profile. The pre-mRNA of OsGolS1 is accumulated in the leaf tissue of etiolated plants at a high level, about fourfold to the accumulation of actin. In one-month-old plants, mature OsGolS1 is found only in leaf sheath Oryza sativa Japonica Group
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2.4.1.123 leaf in one-month-old plants mature OsGolS1 is found only in leaf sheath, OsGolS2 shows both mature and pre-mRNA in sheath. OsGolS2 pre-mRNA is expressed 25fold in leaf sheath Oryza sativa Japonica Group
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2.4.1.123 additional information plant-wide developmental and tissue-specific profile of isozymes OsGolS1 and OsGolS2 functional expression, overview. In 3-month-old plants, mature OsGolS1 is found in late panicle, shoot, and also in root tissue with a precursor Oryza sativa Japonica Group
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2.4.1.123 additional information plant-wide developmental and tissue-specific profile of isozymes OsGolS1 and OsGolS2 functional expression, overview. OsGolS2 pre-mRNA is expressed in all light-grown tissue, ranging from 25fold in leaf sheath to twofold in the root compared to actin. The etiolated plants also show high accumulation of pre-mRNA in roots. In one-month-old plants mature OsGolS1 is found only in leaf sheath, OsGolS2 shows both mature and pre-mRNA in sheath. OsGolS2 is not found in 3-month-old plants Oryza sativa Japonica Group
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2.4.1.123 panicle
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 Oryza sativa Japonica Group
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2.4.1.123 root
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 Oryza sativa Japonica Group
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2.4.1.123 seedling
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 Oryza sativa Japonica Group
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2.4.1.123 shoot
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 Oryza sativa Japonica Group
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2.4.1.123 spikelet
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 Oryza sativa Japonica Group
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.123 UDP-alpha-D-galactose + myo-inositol
-
 Oryza sativa Japonica Group UDP + O-alpha-D-galactosyl-(1->3)-1D-myo-inositol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.123 galactinol synthase
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 Oryza sativa Japonica Group
2.4.1.123 OJ1165_F02.103 locus name Oryza sativa Japonica Group
2.4.1.123 Os07g0687900 locus name Oryza sativa Japonica Group
2.4.1.123 OsGolS1
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 Oryza sativa Japonica Group
2.4.1.123 OsGolS2
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 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
2.4.1.123 Oryza sativa Japonica Group expression of GolS genes is implicated in abiotic stress, especially drought and salinity, overview. A non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene, located in chromosomes 3(OsGolS1) and 7(OsGolS2), are interspersed by conserved introns harboring characteristic premature termination codons (PTC). During abiotic stress, the premature and mature transcripts of both isoforms accumulate in a rhythmic manner for very small time-windows interrupted by phases of complete absence. Reporter gene assay using GolS promoters under abiotic stress does not reflect this accumulation profile, suggesting that this regulation occurs post-transcriptionally. Analysis reveals that a combined transcriptional and post transcriptional control exists in rice to regulate GolS expression under stress. Selective degradation of OsGolS pre-mRNAs additional information

General Information

EC Number General Information Comment Organism
2.4.1.123 metabolism the enzyme catalyzes the first step of galactinol biosynthesis, overview. Galactinol and its adducts of the raffinose family oligosaccharides (RFO) series are implicated in carbon storage, transport, maintaining the source-sink relationship, stress amelioration, and signalling Oryza sativa Japonica Group