Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Feng, K.; Xu, Z.S.; Liu, J.X.; Li, J.W.; Wang, F.; Xiong, A.S.
    Isolation, purification, and characterization of AgUCGalT1, a galactosyltransferase involved in anthocyanin galactosylation in purple celery (Apium graveolens L.) (2018), Planta, 247, 1363-1375 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.294 expressed in Escherichia coli BL21 Apium graveolens
2.4.1.294 expression in Escherichia coli Apium graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.294 0.0092
-
 cyanidin pH 8.0, 30°C Apium graveolens
2.4.1.294 0.0092
-
 cyanidin pH 8.0, 35°C Apium graveolens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.234 Apium graveolens A0A346XR64
-
-
2.4.1.294 Apium graveolens A0A346XR64
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.234
-
 Apium graveolens
2.4.1.294
-
 Apium graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.234 UDP-alpha-D-galactose + cyanidin reaction of EC 2.4.1.294 Apium graveolens UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-alpha-D-galactose + kaempferol 53% of the activity compared to cyanidin Apium graveolens UDP + kaempferol 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-alpha-D-galactose + peonidin 26% of the activity compared to cyanidin Apium graveolens UDP + peonidin 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-alpha-D-galactose + quercetin 25% of the activity compared to cyanidin Apium graveolens UDP + quercetin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 additional information no donors: UDP-glucose and UDP-xylose Apium graveolens ?
-
-
2.4.1.294 UDP-alpha-D-galactose + cyanidin
-
 Apium graveolens UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + kaempferol
-
 Apium graveolens UDP + kaempferol 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + peonidin
-
 Apium graveolens UDP + peonidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-alpha-D-galactose + quercetin
-
 Apium graveolens UDP + quercetin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-D-galactose + peonidin 26% of the activity compared to cyanidin Apium graveolens UDP + peonidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-D-galactose + quercetin 25% of the activity compared to cyanidin Apium graveolens UDP + quercetin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-galactose + cyanidin
-
 Apium graveolens UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
2.4.1.294 UDP-galactose + kaempferol reaction of EC 2.4.1.234, 53% of the activity compared to cyanidin Apium graveolens UDP + kaempferol 3-O-beta-D-galactoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.294 ? x * 49570, calculated from sequence, x * 55000, SDS-PAGE Apium graveolens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.234 UCGalT1
-
 Apium graveolens
2.4.1.234 UDP-galactose:cyanidin 3-O-galactosyltransferase
-
 Apium graveolens
2.4.1.294 DP-galactose:cyanidin 3-O-galactosyltransferase
-
 Apium graveolens
2.4.1.294 UCGalT1
-
 Apium graveolens
2.4.1.294 UDP-galactose:cyanidin 3-O-galactosyltransferase
-
 Apium graveolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.294 35
-
-
 Apium graveolens
2.4.1.294 35
-
 activity with cyanidin and UDP-galactose Apium graveolens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.294 20 45 20°C: about 40% of maximal activity, 45°C: about 60% of maximal activity, activity with cyanidin and UDP-galactose Apium graveolens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.294 6.16
-
 cyanidin pH 8.0, 30°C Apium graveolens
2.4.1.294 6.16
-
 cyanidin pH 8.0, 35°C Apium graveolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.294 8
-
-
 Apium graveolens
2.4.1.294 8
-
 activity with cyanidin and UDP-galactose Apium graveolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.294 7 8.5 pH 7.0: about 40% of maximal activity, pH 8.5: about 20% of maximal activity, activity with cyanidin and UDP-galactose Apium graveolens

Expression

EC Number Organism Comment Expression
2.4.1.294 Apium graveolens expression levels of UCGalT1 are positively correlated with the total anthocyanin contents in purple and non-purple celery varieties additional information

General Information

EC Number General Information Comment Organism
2.4.1.234 metabolism the enzyme is involved in anthocyanin galactosylation in Apium graveolens Apium graveolens
2.4.1.294 metabolism the enzyme is involved in anthocyanin galactosylation in Apium graveolens Apium graveolens