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Literature summary extracted from

  • Inoue, S.; Morita, R.; Kuwata, K.; Kunieda, T.; Ueda, H.; Hara-Nishimura, I.; Minami, Y.
    Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium (2018), Plant Physiol. Biochem., 132, 138-144 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.220 expression in Escherichia coli Persicaria tinctoria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.220 0.74
-
 UDP-glucose pH 10.0, 37°C Persicaria tinctoria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.220 cytosol
-
 Persicaria tinctoria 5829
-
2.4.1.220 endoplasmic reticulum membrane microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes. The enzyme interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate Persicaria tinctoria 5789
-
2.4.1.220 microsome microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes Persicaria tinctoria
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.220 51911
-
 calculated from sequence Persicaria tinctoria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.220 UDP-glucose + indoxyl Persicaria tinctoria the enzyme functions as an indican synthase in plant cells UDP + indican
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.220 Persicaria tinctoria A0A2L2R220
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.220 partial Persicaria tinctoria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.220 leaf in expression analyses PtIGS mRNA is found only in the leaves. It is most highly expressed in the 1st leaves, and decreased with leaf age Persicaria tinctoria
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.220 UDP-glucose + indoxyl
-
 Persicaria tinctoria UDP + indican
-
 ?
2.4.1.220 UDP-glucose + indoxyl the enzyme functions as an indican synthase in plant cells Persicaria tinctoria UDP + indican
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.220 PtIGS
-
 Persicaria tinctoria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.220 45
-
-
 Persicaria tinctoria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.220 10
-
-
 Persicaria tinctoria

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.220 Persicaria tinctoria calculated from sequence
-
 5.39