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Literature summary extracted from

  • Miernyk, J.A.; Randall, D.D.
    Some kinetic and regulatory properties of the pea mitochondrial pyruvate dehydrogenase complex (1987), Plant Physiol., 83, 306-310 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 acetyl-CoA
-
 Pisum sativum
1.2.1.104 glyoxylate inhibitory only in the absence of thiol reagents, competitive with respect to pyruvate Pisum sativum
1.2.1.104 NADH
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.104 0.0046
-
 CoA pH 7.6, 22°C Pisum sativum
1.2.1.104 0.0567
-
 pyruvate pH 7.6, 22°C Pisum sativum
1.2.1.104 0.1217
-
 NAD+ pH 7.6, 22°C Pisum sativum
1.2.1.104 1.35
-
 2-oxobutanoate pH 7.6, 22°C Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.104 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.104 Ca2+ 81% of the activity with Mg2+ Pisum sativum
1.2.1.104 Co2+ 4% of the activity with Mg2+ Pisum sativum
1.2.1.104 Mg2+ divalent cation requirement is best satisfied by Mg2+ Pisum sativum
1.2.1.104 Mn2+ 89% of the activity with Mg2+ Pisum sativum
1.2.1.104 additional information divalent cation requirement is best satisfied by Mg2+ Pisum sativum
1.2.1.104 Ni2+ 62% of the activity with Mg2+ Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Pisum sativum P52904 and P52902 P52904 i.e. E1 component subunit beta, P52902 i.e. E1 component subunit alpha
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 2-oxobutanoate + CoA + NAD+
-
 Pisum sativum propanoyl-CoA + CO2 + NADH
-
 ?
1.2.1.104 additional information pyruvate is a better substrate than 2-oxobutanoate, other 2-oxo-acids are not substrates Pisum sativum ?
-
-
1.2.1.104 pyruvate + CoA + NAD+
-
 Pisum sativum acetyl-CoA + CO2 + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.104 40
-
-
 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.104 7.6
-
 overall reaction Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.104 0.0098
-
 acetyl-CoA pH 7.6, 22°C Pisum sativum
1.2.1.104 0.0182
-
 NADH pH 7.6, 22°C Pisum sativum
1.2.1.104 0.051
-
 glyoxylate pH 7.6, 22°C Pisum sativum