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Literature summary extracted from

  • Sun, J.; Sigler, C.; Beaudoin, G.; Joshi, J.; Patterson, J.; Cho, K.; Ralat, M.; Gregory, J.; Clark, D.; Deng, Z.; Colquhoun, T.; Hanson, A.
    Parts-prospecting for a high-efficiency thiamin thiazole biosynthesis pathway (2019), Plant Physiol., 179, 958-968 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.59 expressed in Escherichia coli DELTAthiG strain Thermovibrio ammonificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.60 chloroplast
-
Caladium bicolor 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.59 NAD+ + glycine + sulfide Thermovibrio ammonificans
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Caladium bicolor
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.59 Thermovibrio ammonificans
-
-
-
2.4.2.60 Caladium bicolor A0A3G2LN21 cultivar Tapestry
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.60 flower the enzyme is massively expressed in male flower Caladium bicolor
-
2.4.2.60 leaf
-
Caladium bicolor
-
2.4.2.60 additional information not expressed in roots Caladium bicolor
-
2.4.2.60 receptacle
-
Caladium bicolor
-
2.4.2.60 spathe
-
Caladium bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.59 NAD+ + glycine + sulfide
-
Thermovibrio ammonificans nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
Caladium bicolor nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.59 Thi4
-
Thermovibrio ammonificans
2.4.2.60 Thi4
-
Caladium bicolor