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Literature summary extracted from

  • Sun, G.; Strebl, M.; Merz, M.; Blamberg, R.; Huang, F.C.; McGraphery, K.; Hoffmann, T.; Schwab, W.
    Glucosylation of the phytoalexin N-feruloyl tyramine modulates the levels of pathogen-responsive metabolites in Nicotiana benthamiana (2019), Plant J., 100, 20-37 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.128 DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A24 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates Nicotiana benthamiana
2.4.1.128 DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A25 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates Nicotiana benthamiana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.128 additional information overexpression of UGT73A24 and UGT73A25 leads to a significant reduction in the level of the pathogen-responsive metabolites, including phenylalanine and tryptophan although the latter are not substrates of the encoded enzymes Nicotiana benthamiana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.128 additional information
-
 additional information Michaelis-Menten kinetics Nicotiana benthamiana
2.4.1.128 0.0022
-
 kaempferol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.0035
-
 quercetin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.0043
-
 quercetin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.0187
-
 kaempferol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.1103
-
 N-4-trans-coumaroyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.1505
-
 N-caffeoyl-O-methyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.1592
-
 N-caffeoyl-O-methyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.2826
-
 N-4-trans-coumaroyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.3245
-
 scopoletin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.8818
-
 carvacrol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.9123
-
 scopoletin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 1.2721
-
 carvacrol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 1.4732
-
 N-trans-feruloyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 3.0307
-
 N-trans-feruloyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.128 additional information Nicotiana benthamiana UGT73A25 appears to promote the formation of flavonoid rutinosides and a kaempferol diglucoside, while UGT73A24 does not ?
-
 ?
2.4.1.128 UDP-glucose + kaempferol Nicotiana benthamiana best substrate UDP + ?
-
 ?
2.4.1.128 UDP-glucose + kaempferol Nicotiana benthamiana
-
 UDP + kaempferol 3,7-diglucoside
-
 ?
2.4.1.128 UDP-glucose + N-trans-feruloyl tyramine Nicotiana benthamiana
-
 UDP + ?
-
 ?
2.4.1.128 UDP-glucose + scopoletin Nicotiana benthamiana
-
 UDP + scopolin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.128 Nicotiana benthamiana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.128 callus
-
 Nicotiana benthamiana
-
2.4.1.128 leaf
-
 Nicotiana benthamiana
-
2.4.1.128 root
-
 Nicotiana benthamiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.128 additional information UGT73A25 appears to promote the formation of flavonoid rutinosides and a kaempferol diglucoside, while UGT73A24 does not Nicotiana benthamiana ?
-
 ?
2.4.1.128 additional information broad substrate specificity of NbUGT73A25, detailed overview. Enzymes UGT73A24 and UGT73A25 show the highest activity towards kaempferol Nicotiana benthamiana ?
-
 ?
2.4.1.128 UDP-glucose + carvacrol
-
 Nicotiana benthamiana UDP + carvacrol
-
 ?
2.4.1.128 UDP-glucose + kaempferol best substrate Nicotiana benthamiana UDP + ?
-
 ?
2.4.1.128 UDP-glucose + kaempferol
-
 Nicotiana benthamiana UDP + kaempferol 3,7-diglucoside
-
 ?
2.4.1.128 UDP-glucose + kaempferol best substrate Nicotiana benthamiana UDP + kaempferol 3,7-diglucoside
-
 ?
2.4.1.128 UDP-glucose + N-4-trans-coumaroyl tyramine
-
 Nicotiana benthamiana UDP + ?
-
 ?
2.4.1.128 UDP-glucose + N-caffeoyl-O-methyl tyramine
-
 Nicotiana benthamiana UDP + ?
-
 ?
2.4.1.128 UDP-glucose + N-trans-feruloyl tyramine
-
 Nicotiana benthamiana UDP + ?
-
 ?
2.4.1.128 UDP-glucose + quercetin
-
 Nicotiana benthamiana UDP + ?
-
 ?
2.4.1.128 UDP-glucose + scopoletin
-
 Nicotiana benthamiana UDP + scopolin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.128 N-feruloyltyramine glucosyltransferase
-
 Nicotiana benthamiana
2.4.1.128 NbUGT73A24
-
 Nicotiana benthamiana
2.4.1.128 NbUGT73A25
-
 Nicotiana benthamiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.128 30
-
 assay at Nicotiana benthamiana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.128 0.01
-
 N-4-trans-coumaroyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.06
-
 N-caffeoyl-O-methyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.06
-
 N-trans-feruloyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.07
-
 N-caffeoyl-O-methyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.264
-
 quercetin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.341
-
 kaempferol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.589
-
 kaempferol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.65
-
 quercetin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.766
-
 scopoletin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.811
-
 carvacrol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.916
-
 scopoletin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.967
-
 carvacrol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.128 7.5
-
 assay at Nicotiana benthamiana

General Information

EC Number General Information Comment Organism
2.4.1.128 evolution Nicotiana benthamiana enzymes UGT73A24 and UGT73A25 show 95% sequence identity Nicotiana benthamiana
2.4.1.128 metabolism while in Nicotiana tabacum, TOGT (tobacco UGT) glucosylates scopoletin after treatment with salicylate, fungal elicitors and the tobacco mosaic virus, enzymes NbUGT73A24 and NbUGT73A25 produce glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana Nicotiana benthamiana
2.4.1.128 physiological function NbUGT73A24 produces glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana Nicotiana benthamiana
2.4.1.128 physiological function NbUGT73A25 produces glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana Nicotiana benthamiana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.128 0.02
-
 N-trans-feruloyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.035
-
 N-4-trans-coumaroyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.041
-
 N-trans-feruloyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.09
-
 N-4-trans-coumaroyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.4
-
 N-caffeoyl-O-methyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.44
-
 N-caffeoyl-O-methyl tyramine recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.76
-
 carvacrol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.84
-
 scopoletin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 0.92
-
 carvacrol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 2.82
-
 scopoletin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 31.5
-
 kaempferol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 61.4
-
 quercetin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 155
-
 kaempferol recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana
2.4.1.128 185.71
-
 quercetin recombinant enzyme, pH 7.5, 30°C Nicotiana benthamiana