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Literature summary extracted from

  • Witasari, L.D.; Huang, F.C.; Hoffmann, T.; Rozhon, W.; Fry, S.C.; Schwab, W.
    Higher expression of the strawberry xyloglucan endotransglucosylase/hydrolase genes FvXTH9 and FvXTH6 accelerates fruit ripening (2019), Plant J., 100, 1237-1253 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B52 phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH9 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH6, recombinant expression in Nicotiana tabacum cv. Samsum Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells Fragaria vesca
2.4.1.207 phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH6 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH9, recombinant expression in Nicotiana tabacum cv. Samsum Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells Fragaria vesca
2.4.1.207 phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH9 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH6, recombinant expression in Nicotiana tabacum cv. Samsum via Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells Fragaria vesca

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B52 additional information Fragaria x ananassa cv. Elsanta fruits infiltrated with FvXTH9 and FvXTH6 ripen faster and show decreased firmness compared with the empty vector control pBI121 Fragaria vesca
2.4.1.207 additional information Fragaria x ananassa cv. Elsanta fruits infiltrated with FvXTH9 and FvXTH6 ripen faster and show decreased firmness compared with the empty vector control pBI121 Fragaria vesca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.207 additional information
-
 additional information Michaelis-Menten kinetics Fragaria vesca

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.B52 additional information prediction of subcellular localization suggests a localization to the secretory pathway Fragaria vesca
-
-
2.4.1.207 additional information prediction of subcellular localization suggests a localization to the secretory pathway Fragaria vesca
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B52 Fragaria vesca
-
 cv. Hawaii 4
-
2.4.1.207 Fragaria vesca
-
 cv. Hawaii 4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.B52 recombinant His-tagged enzyme from Saccharomyces cerevisiae INVSc1 cells by nickel affinity chromatography Fragaria vesca
2.4.1.207 recombinant His-tagged enzyme from Saccharomyces cerevisiae INVSc1 cells by nickel affinity chromatography Fragaria vesca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.B52 flower high expression level of FvXTH9 Fragaria vesca
-
2.4.1.B52 fruit FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages Fragaria vesca
-
2.4.1.B52 additional information to confirm the transcriptome data, the expression pattern of FvXTH9 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages. A high expression level of FvXTH9 is also determined in flowers, whereas its mRNA abundance is very low in all other tissues investigated. The expression pattern of FvXTH6 during fruit development resembled that of FvXTH9. FvXTH9 shows the highest expression level in green receptacles Fragaria vesca
-
2.4.1.207 flower high expression level of FvXTH6 Fragaria vesca
-
2.4.1.207 flower high expression level of FvXTH9 Fragaria vesca
-
2.4.1.207 fruit FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages Fragaria vesca
-
2.4.1.207 fruit the expression pattern of FvXTH6 during fruit development shows the highest level in fully developed green fruit and with a clear decline in later stages Fragaria vesca
-
2.4.1.207 leaf FvXTH6 is expressed at a relatively high level in young leaf tissue but at a low level in old leaf tissue Fragaria vesca
-
2.4.1.207 additional information to confirm the transcriptome data, the expression pattern of FvXTH6 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. The expression pattern of FvXTH6 during fruit development shows the highest level in fully developed green fruit and with a clear decline in later stages. The absolute expression level of FvXTH6 in fruit is much lower than that of FvXTH9. FvXTH6 is also highly expressed in flowers. FvXTH6 is expressed at relatively high level in young leaf tissue but at low level in old leaf tissue Fragaria vesca
-
2.4.1.207 additional information to confirm the transcriptome data, the expression pattern of FvXTH9 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages. A high expression level of FvXTH9 is also determined in flowers, whereas its mRNA abundance is very low in all other tissues investigated. The expression pattern of FvXTH6 during fruit development resembled that of FvXTH9. FvXTH9 shows the highest expression level in green receptacles Fragaria vesca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B52 barley (1->3,1->4)-beta-D-glucan + XLLGol MXE activity Fragaria vesca ?
-
 ?
2.4.1.B52 barley (1->3,1->4)-beta-D-glucan + XXFGol MXE activity Fragaria vesca ?
-
 ?
2.4.1.B52 barley (1->3,1->4)-beta-D-glucan + XXGol MXE activity Fragaria vesca ?
-
 ?
2.4.1.B52 barley (1->3,1->4)-beta-D-glucan + XXLGol MXE activity Fragaria vesca ?
-
 ?
2.4.1.B52 barley (1->3,1->4)-beta-D-glucan + XXXGol MXE activity Fragaria vesca ?
-
 ?
2.4.1.B52 hydroxyethylcellulose + XXXGol CXE activity Fragaria vesca ?
-
 ?
2.4.1.B52 additional information enzyme FvXTH9 shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE), cellulose:xyloglucan endotransglucosylase (CXE), and xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activities Fragaria vesca ?
-
-
2.4.1.207 additional information enzyme FvXTH9 shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE, EC 2.4.1.B52), cellulose:xyloglucan endotransglucosylase (CXE, EC 2.4.1.B52), and xyloglucan endotransglucosylase (XET) activities Fragaria vesca ?
-
-
2.4.1.207 tamarind xyloglucan + XLLGol XET activity Fragaria vesca ?
-
 ?
2.4.1.207 tamarind xyloglucan + XXFGol XET activity Fragaria vesca ?
-
 ?
2.4.1.207 tamarind xyloglucan + XXGol XET activity Fragaria vesca ?
-
 ?
2.4.1.207 tamarind xyloglucan + XXLGol XET activity Fragaria vesca ?
-
 ?
2.4.1.207 tamarind xyloglucan + XXXGol XET activity Fragaria vesca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B52 ? x * 33200, about, sequence calculation Fragaria vesca
2.4.1.207 ? x * 33200, about, sequence calculation Fragaria vesca

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B52 cellulose:xyloglucan endotransglucosylase
-
 Fragaria vesca
2.4.1.B52 CXE
-
 Fragaria vesca
2.4.1.B52 FvXTH9
-
 Fragaria vesca
2.4.1.B52 mixed-linkage glucan:xyloglucan endotransglucosylase
-
 Fragaria vesca
2.4.1.B52 More see also EC 2.4.1.207 Fragaria vesca
2.4.1.B52 MXE
-
 Fragaria vesca
2.4.1.B52 XTH9
-
 Fragaria vesca
2.4.1.207 FvXTH6
-
 Fragaria vesca
2.4.1.207 FvXTH9
-
 Fragaria vesca
2.4.1.207 More see also EC 2.4.1.B52 Fragaria vesca
2.4.1.207 XET
-
 Fragaria vesca
2.4.1.207 XTH6
-
 Fragaria vesca
2.4.1.207 XTH9
-
 Fragaria vesca
2.4.1.207 xyloglucan endotransglucosylase
-
 Fragaria vesca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B52 25
-
 assay at Fragaria vesca
2.4.1.207 25
-
 assay at Fragaria vesca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B52 5.5
-
 CXE activity Fragaria vesca
2.4.1.B52 6.5
-
 MXE activity Fragaria vesca
2.4.1.207 6.5
-
-
 Fragaria vesca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.B52 Fragaria vesca sequence calculation
-
 5.46
2.4.1.207 Fragaria vesca sequence calculation
-
 5.46

General Information

EC Number General Information Comment Organism
2.4.1.B52 evolution XTHs are classified together with the lichenases, which hydrolyse mixed-linkage (1->3,1->4)-beta-D-glucans (MLG), in glycoside hydrolase family 16 Fragaria vesca
2.4.1.B52 metabolism enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE). XTHs (xyloglucan endotransglucosylases/hydrolases) can catalyse the endolytic cleavage of xyloglucan polymers and the rejoining of the newly generated reducing ends to other xyloglucan molecules, which is referred to as xyloglucan endotransglucosylase (XET) activity. In addition, XTHs can also show xyloglucan endohydrolase (XEH) activity, where water is used as an acceptor, and thus the xyloglucan molecule is hydrolysed Fragaria vesca
2.4.1.B52 physiological function FvXTH9 and also FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid Fragaria vesca
2.4.1.207 evolution XTHs are classified together with the lichenases, which hydrolyse mixed-linkage (1->3,1->4)-beta-D-glucans (MLG), in glycoside hydrolase family 16 Fragaria vesca
2.4.1.207 metabolism enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE) Fragaria vesca
2.4.1.207 metabolism enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE). XTHs (xyloglucan endotransglucosylases/hydrolases) can catalyse the endolytic cleavage of xyloglucan polymers and the rejoining of the newly generated reducing ends to other xyloglucan molecules, which is referred to as xyloglucan endotransglucosylase (XET) activity. In addition, XTHs can also show xyloglucan endohydrolase (XEH) activity, where water is used as an acceptor, and thus the xyloglucan molecule is hydrolysed Fragaria vesca
2.4.1.207 physiological function FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid Fragaria vesca
2.4.1.207 physiological function FvXTH9 and also FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid Fragaria vesca