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Literature summary extracted from

  • Lee, J.; Kim, Y.; Jung, Y.; Han, J.; Zhang, C.; Yun, C.; Lee, S.
    The overexpression of cucumber (Cucumis sativus L.) genes that encode the branched-chain amino acid transferase modulate flowering time in Arabidopsis thaliana (2019), Plant Cell Rep., 38, 25-35 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 locus name Csa2G020990, localization on chromosome 2: 2526524-2536725, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Arabidosis thaliana ecotype Col-0, quantitative isozyme expression analysis, recombinant expression of GFP-tagged isozyme in Cucumis sativus protoplasts (35S::F1 ATPase N77:GFP is used as a mitochondrial targeting control), recombinant expression of His-tagged isozyme in Escherichia coli strain Rosetta(DE3) Cucumis sativus var. sativus
2.6.1.42 locus name Csa2G352930, localization on chromosome 2: 16313915-16315681, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Arabidosis thaliana ecotype Col-0, quantitative isozyme expression analysis, recombinant expression of GFP-tagged isozyme in Cucumis sativus protoplasts (35S::F1 ATPase N77:GFP is used as a mitochondrial targeting control), recombinant expression of His-tagged isozyme in Escherichia coli strain Rosetta(DE3) Cucumis sativus var. sativus
2.6.1.42 locus name Csa6G496970, localization on chromosome 6: 24245649-24253151, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Arabidosis thaliana ecotype Col-0, quantitative isozyme expression analysis, recombinant expression of GFP-tagged isozyme in Cucumis sativus protoplasts (35S::F1 ATPase N77:GFP is used as a mitochondrial targeting control), recombinant expression of His-tagged isozyme in Escherichia coli strain Rosetta(DE3) Cucumis sativus var. sativus

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.42 additional information phenotypic analysis of 35S::CsBCAT2 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana Cucumis sativus var. sativus
2.6.1.42 additional information phenotypic analysis of 35S::CsBCAT3 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana Cucumis sativus var. sativus
2.6.1.42 additional information phenotypic analysis of 35S::CsBCAT7 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana Cucumis sativus var. sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 mitochondrion
-
 Cucumis sativus var. sativus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Cucumis sativus var. sativus
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Cucumis sativus var. sativus
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Cucumis sativus var. sativus
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Cucumis sativus var. sativus A0A0A0KG15 monoecious Cucumis sativus L. var. sativus cv. WJE F10, and gynoecious Cucumis sativus L. var. sativus cv. WNE F8
-
2.6.1.42 Cucumis sativus var. sativus A0A0A0LJI6 monoecious Cucumis sativus L. var. sativus cv. WJE F10, and gynoecious Cucumis sativus L. var. sativus cv. WNE F8
-
2.6.1.42 Cucumis sativus var. sativus A0A0A0LN48 monoecious Cucumis sativus L. var. sativus cv. WJE F10, and gynoecious Cucumis sativus L. var. sativus cv. WNE F8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 recombinant His-tagged isozyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography Cucumis sativus var. sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.42 cotyledon
-
 Cucumis sativus var. sativus
-
2.6.1.42 flower
-
 Cucumis sativus var. sativus
-
2.6.1.42 leaf
-
 Cucumis sativus var. sativus
-
2.6.1.42 additional information isozymes CsBCAT2 and CsBCAT7 are highly expressed in the reproductive tissues, whereas isozyme CsBCAT3 expression is highly detected in the vegetative tissues, spatial expression profiling of the three BCAT genes in cucumber, overview Cucumis sativus var. sativus
-
2.6.1.42 root
-
 Cucumis sativus var. sativus
-
2.6.1.42 stem
-
 Cucumis sativus var. sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Cucumis sativus var. sativus 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Cucumis sativus var. sativus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Cucumis sativus var. sativus 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
 Cucumis sativus var. sativus
2.6.1.42 branched-chain amino acid transferase
-
 Cucumis sativus var. sativus
2.6.1.42 CsBCAT2
-
 Cucumis sativus var. sativus
2.6.1.42 CsBCAT3
-
 Cucumis sativus var. sativus
2.6.1.42 CsBCAT7
-
 Cucumis sativus var. sativus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Cucumis sativus var. sativus
2.6.1.42 pyridoxal 5'-phosphate PLP Cucumis sativus var. sativus

General Information

EC Number General Information Comment Organism
2.6.1.42 malfunction isozymes CsBCAT2 and CsBCAT3 restore the growth of a bat1DELTA/bat2DELTA double knockout Saccharomyces cerevisiae strain, and isozymes CsBCAT3 and CsBCAT7, that show different substrate preferences, act in a reverse reaction. The transgenic approach demonstrates that the overexpression of the three CsBCATs results in early flowering phenotypes, which are associated with the upregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) in a manner in which they are dependent on GIGANTEA (GI)/CONSTANS (CO) and SHORT VEGETATIVE PHASE (SVP)/FLOWERING LOCUS C (FLC) modules. The overexpressed CsBCAT isozymes with BCAT enzymatic activities affect the flowering time in transgenic Arabidopsis thaliana Cucumis sativus var. sativus
2.6.1.42 physiological function the BCAT enzymes catalyze the interconversion of BCAAs to the branched-chain keto acids (BCKAs). There is an interconnection between branched-chain amino acid transferases (BCAT) genes, that function in branched-chain amino acid (BCAA) metabolism, and the flowering time in plants. The overexpressed CsBCAT isozymes with BCAT enzymatic activities affect the flowering time in transgenic Arabidopsis thaliana Cucumis sativus var. sativus