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Literature summary extracted from

  • Ivanov Kavkova, E.; Bloechl, C.; Tenhaken, R.
    The Myo-inositol pathway does not contribute to ascorbic acid synthesis (2019), Plant Biol., 21 Suppl 1, 95-102 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.43 additional information generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.43 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.43 ATP + D-glucuronate Arabidopsis thaliana
-
 ADP + 1-phospho-alpha-D-glucuronate
-
 ?
2.7.1.43 ATP + D-glucuronate Arabidopsis thaliana Col-0
-
 ADP + 1-phospho-alpha-D-glucuronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.43 Arabidopsis thaliana Q93ZC9
-
-
2.7.1.43 Arabidopsis thaliana Col-0 Q93ZC9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.43 leaf
-
 Arabidopsis thaliana
-
2.7.1.43 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.43 ATP + D-glucuronate
-
 Arabidopsis thaliana ADP + 1-phospho-alpha-D-glucuronate
-
 ?
2.7.1.43 ATP + D-glucuronate
-
 Arabidopsis thaliana Col-0 ADP + 1-phospho-alpha-D-glucuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.43 At3g01640
-
 Arabidopsis thaliana
2.7.1.43 GlcAK1
-
 Arabidopsis thaliana
2.7.1.43 glucuronokinase1
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.43 malfunction GlcAK1 knockout mutant phenotype of T-DNA insertional mutants glcak1-1, glcak1-2, glcak1-3, and glcak1-4, overview Arabidopsis thaliana
2.7.1.43 metabolism one alternative pathway for ascorbic acid (AsA) synthesis, which is similar to the biosynthesis route in mammals, is controversially discussed for plants. Pathway overview for AsA biosynthesis in plants, overview. Here, myo-inositol is cleaved to glucuronic acid and then converted via L-gulonate to AsA. In contrast to animals, plants have an effective recycling pathway for glucuronic acid, being a competitor for the metabolic rate. Recycling involves a phosphorylation at C1 by the enzyme glucuronokinase Arabidopsis thaliana