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Literature summary extracted from

  • Hamed, R.; Maharem, T.; Guneidy, R.; Emam, M.; Abdel Karim, G.
    Purification of fat body glutathione S-transferase from the desert locust Schistocerca gregaria investigation of flavonoid inhibitory effects on enzyme activity (2019), Physiol. Entomol., 44, 187-199 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 bromosulfophthalein
-
 Schistocerca gregaria
2.5.1.18 Cibacron blue most potent inhibitor Schistocerca gregaria
2.5.1.18 delphinidin chloride strong inhibition Schistocerca gregaria
2.5.1.18 Ethacrynic acid
-
 Schistocerca gregaria
2.5.1.18 additional information not inhibited by 18alpha-glycyrrhetinic acid and cyanidin Schistocerca gregaria
2.5.1.18 quercetin strong inhibition Schistocerca gregaria
2.5.1.18 rutin
-
 Schistocerca gregaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.2
-
 1-chloro-2,4-dinitrobenzene at pH 8.0 and 30°C Schistocerca gregaria
2.5.1.18 0.354
-
 glutathione at pH 8.0 and 30°C Schistocerca gregaria

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Schistocerca gregaria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 DEAE-Sepharose column chromatography and GSH-Sepharose column chromatography Schistocerca gregaria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 fat body highest enzyme activity in fat body Schistocerca gregaria
-
2.5.1.18 foregut
-
 Schistocerca gregaria
-
2.5.1.18 hindgut
-
 Schistocerca gregaria
-
2.5.1.18 midgut
-
 Schistocerca gregaria
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 2.66
-
 crude extract, at pH 8.0 and 30°C Schistocerca gregaria
2.5.1.18 42.8
-
 after 16.1fold purification, at pH 8.0 and 30°C Schistocerca gregaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Schistocerca gregaria ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Schistocerca gregaria chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide 114% activity compared to 1-chloro-2,4-dinitrobenzene Schistocerca gregaria ?
-
 ?
2.5.1.18 additional information the enzyme has no detectable activity toward 1,2-dichloro-4-nitrobenzene, ethacrynic acid, bromosulfophthalein, 1,2-epoxy-3-(4-nitrophenoxy) propane, 4-nitrophenethyl bromide and styrene oxide Schistocerca gregaria ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 24000, SDS-PAGE Schistocerca gregaria
2.5.1.18 ? x * 23600, calculated from amino acid sequence Schistocerca gregaria

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Schistocerca gregaria
2.5.1.18 GST
-
 Schistocerca gregaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 8
-
 optimum at pH 8.0 followed by an approximately 70% decrease in enzymatic activity in alkaline pH values Schistocerca gregaria

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.000189
-
 at pH 8.0 and 30°C Schistocerca gregaria Cibacron blue
2.5.1.18 0.00223
-
 at pH 8.0 and 30°C Schistocerca gregaria quercetin
2.5.1.18 0.00388
-
 at pH 8.0 and 30°C Schistocerca gregaria delphinidin chloride
2.5.1.18 0.0103
-
 at pH 8.0 and 30°C Schistocerca gregaria rutin
2.5.1.18 0.363
-
 at pH 8.0 and 30°C Schistocerca gregaria bromosulfophthalein
2.5.1.18 3.63
-
 at pH 8.0 and 30°C Schistocerca gregaria Ethacrynic acid