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Literature summary extracted from

  • Zhou, K.; Wunsch, C.; Dai, J.; Li, S.M.
    gem-Diprenylation of acylphloroglucinols by a fungal prenyltransferase of the dimethylallyltryptophan synthase superfamily (2017), Org. Lett., 19, 388-391 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.136 expressed in Escherichia coli M15 [pREP] cells Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.136 Co2+ strong inhibition at 5 mM Aspergillus terreus
2.5.1.136 Cu2+ complete inhibition at 5 mM Aspergillus terreus
2.5.1.136 Fe2+ strong inhibition at 5 mM Aspergillus terreus
2.5.1.136 Mn2+ complete inhibition at 5 mM Aspergillus terreus
2.5.1.136 Ni2+ complete inhibition at 5 mM Aspergillus terreus
2.5.1.136 Zn2+ almost complete inhibition at 5 mM Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.136 0.071
-
 dimethylallyl diphosphate with 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.16
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.38
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.42
-
 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.46
-
 geranyl diphosphate with phenyl(2,4,6-trihydroxyphenyl)methanone as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.47
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with geranyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.7
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.136 Ca2+ stimulates activity, 5 mM used in assay conditions Aspergillus terreus
2.5.1.136 Mg2+ stimulates activity Aspergillus terreus
2.5.1.136 additional information not influenced by K+ and Na+ Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.136 Aspergillus terreus A0A1B0UHJ4
-
-
2.5.1.136 Aspergillus terreus DSM 1958 A0A1B0UHJ4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.136 dimethylallyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 Aspergillus terreus diphosphate + 3,5-dihydroxy-4,4-bis(3-methylbut-2-en-1-yl)-2-(2-methylpropanoyl)cyclohexa-2,5-dien-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 3,5-dihydroxy-4,4-bis(3-methylbut-2-en-1-yl)-2-(2-methylpropanoyl)cyclohexa-2,5-dien-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 Aspergillus terreus diphosphate + 3,5-dihydroxy-2-(3-methylbutanoyl)-4,4-bis(3-methylbut-2-en-1-yl)cyclohexa-2,5-dien-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 3,5-dihydroxy-2-(3-methylbutanoyl)-4,4-bis(3-methylbut-2-en-1-yl)cyclohexa-2,5-dien-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus diphosphate + phenyl[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]methanone
-
 ?
2.5.1.136 dimethylallyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus DSM 1958 diphosphate + phenyl[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]methanone
-
 ?
2.5.1.136 dimethylallyl diphosphate + phenyl[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]methanone
-
 Aspergillus terreus diphosphate + 2-benzoyl-3,5-dihydroxy-4,4-bis(3-methylbut-2-en-1-yl)cyclohexa-2,5-dien-1-one
-
 ?
2.5.1.136 farnesyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]phenyl]propan-1-one
-
 ?
2.5.1.136 farnesyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]phenyl]butan-1-one
-
 ?
2.5.1.136 farnesyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus diphosphate + phenyl[2,4,6-trihydroxy-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]phenyl]methanone
-
 ?
2.5.1.136 geranyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus diphosphate + 1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4,6-trihydroxyphenyl]-2-methylpropan-1-one
-
 ?
2.5.1.136 geranyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus diphosphate + 1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4,6-trihydroxyphenyl]-3-methylbutan-1-one
-
 ?
2.5.1.136 geranyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus diphosphate + [3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4,6-trihydroxyphenyl](phenyl)methanone
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.136 ? x * 45000, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.136 apT
-
 Aspergillus terreus
2.5.1.136 aromatic prenyltransferase
-
 Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.136 0.012
-
 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.012
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.014
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.126
-
 geranyl diphosphate with 3 as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.126
-
 geranyl diphosphate with phenyl(2,4,6-trihydroxyphenyl)methanone as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.137
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with geranyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.27
-
 dimethylallyl diphosphate with 1 as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.27
-
 dimethylallyl diphosphate with 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.284
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.136 0.0171
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.0286
-
 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.0368
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.2739
-
 geranyl diphosphate with phenyl(2,4,6-trihydroxyphenyl)methanone as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 0.2915
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with geranyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 1.775
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
2.5.1.136 3.8169
-
 dimethylallyl diphosphate with 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus