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Literature summary extracted from

  • Lowe, P.T.; Cobb, S.L.; OHagan, D.
    An enzymatic Finkelstein reaction fluorinase catalyses direct halogen exchange (2019), Org. Biomol. Chem., 17, 7493-7496 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.0097
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya
2.5.1.94 0.0097
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya Q70GK9 bifunctional enzyme, catalyzes both the reaction of fluorinase EC 2.5.1.63 and chlorinase Ec 2.5.1.94
-
2.5.1.94 Streptomyces cattleya Q70GK9 bifunctional enzyme, catalyzes both the reaction of fluorinase EC 2.5.1.63 and chlorinase Ec 2.5.1.94
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 2'-deoxyadenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 2'-deoxy-5'-fluoroadenosine + L-methionine
-
 r
2.5.1.63 5-deoxy-5'-bromoadenosine + fluoride
-
 Streptomyces cattleya 5-deoxy-5'-fluoroadenosine + bromide
-
 r
2.5.1.63 5-deoxy-5'-chloroadenosine + L-methionine
-
 Streptomyces cattleya S-adenosyl-L-methionine + chloride
-
 r
2.5.1.63 5-deoxy-5'-iodoadenosine + fluoride
-
 Streptomyces cattleya 5-deoxy-5'-fluoroadenosine + iodide
-
 r
2.5.1.94 2'-deoxyadenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 2'-deoxy-5'-fluoroadenosine + L-methionine
-
 r
2.5.1.94 5-deoxy-5'-bromoadenosine + fluoride
-
 Streptomyces cattleya 5-deoxy-5'-fluoroadenosine + bromide
-
 r
2.5.1.94 5-deoxy-5'-chloroadenosine + L-methionine
-
 Streptomyces cattleya S-adenosyl-L-methionine + chloride
-
 r
2.5.1.94 5-deoxy-5'-iodoadenosine + fluoride
-
 Streptomyces cattleya 5-deoxy-5'-fluoroadenosine + iodide
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.63 0.0003
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya
2.5.1.94 0.0003
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.63 0.035
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya
2.5.1.94 0.035
-
 5-deoxy-5'-bromoadenosine 37°C, direct displacement reaction in the absence of L-methionine or S-adenosyl-L-methionine, pH not specified in the publication Streptomyces cattleya