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Literature summary extracted from

  • Chen, Y.; Brooks, A.; Goodenough-Lashua, D.; Kittendorf, J.; Showalter, H.; Garcia, G.
    Evolution of eukaryal tRNA-guanine transglycosylase Insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases (2011), Nucleic Acids Res., 39, 2834-2844 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.64
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.64 V161C the mutant enzyme exhibits a significant improvement in preQ1 (7-aminomethyl-7-deazaguanine) recognition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.64 0.00026
-
 queuine pH 7.3, 37°C, wild-type enzyme Homo sapiens
2.4.2.64 0.0223
-
 7-aminomethyl-7-carbaguanine pH 7.3, 37°C, mutant enzyme V161C Homo sapiens
2.4.2.64 0.132
-
 7-aminomethyl-7-carbaguanine pH 7.3, 37°C, wild-type enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.64 Homo sapiens Q9BXR0 AND Q9H974 Q9BXR0: catalytic subunit 1 (QTRT1), Q9H974: accessory subunit 2 (QTRT2)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.64
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.64 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine very low activity with wild-type enzyme. The mutant enzyme V161C exhibits a significant improvement in preQ1 (7-aminomethyl-7-deazaguanine) recognition Homo sapiens 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?
2.4.2.64 guanine34 in tRNA + queuine
-
 Homo sapiens queuine34 in tRNA + guanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.64 QTRT1 gene name, catalytic subunit 1 Homo sapiens
2.4.2.64 QTRT2 gene name, accessory subunit 2 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.64 0.00464
-
 7-aminomethyl-7-carbaguanine pH 7.3, 37°C, mutant enzyme V161C Homo sapiens
2.4.2.64 0.00822
-
 queuine pH 7.3, 37°C, wild-type enzyme Homo sapiens
2.4.2.64 0.00823
-
 7-aminomethyl-7-carbaguanine pH 7.3, 37°C, wild-type enzyme Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.2.64 evolution present classes of tRNA-guanine transglycosylase have arisen via divergent, not convergent, evolution Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.64 0.062
-
 7-aminomethyl-7-carbaguanine pH 7.3, 37°C, wild-type enzyme Homo sapiens
2.4.2.64 0.21
-
 7-aminomethyl-7-carbaguanine pH 7.3, 37°C, mutant enzyme V161C Homo sapiens
2.4.2.64 31.6
-
 queuine pH 7.3, 37°C, wild-type enzyme Homo sapiens