Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sanchez-Moreno, I.; Trachtmann, N.; Ilhan, S.; Helaine, V.; Lemaire, M.; Guerard-Helaine, C.; Sprenger, G.A.
    2-Ketogluconate kinase from Cupriavidusnecator H16 purification, characterization, and exploration of its substrate specificity (2019), Molecules, 24, 2393 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.13 expression in Escherichia coli BL21(DE3) Cupriavidus necator
2.7.1.13 gene kguK, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Cupriavidus necator
2.7.1.45 gene kguK, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Cupriavidus necator

General Stability

EC Number General Stability Organism
2.7.1.13 the enzyme is stabilized by storage in an imidazole solution which is removed just before use Cupriavidus necator
2.7.1.13 the enzyme is unstable in its pure form Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.13 additional information
-
 additional information steady-state kinetics of the recombinant enzyme Cupriavidus necator
2.7.1.13 0.073
-
 ATP pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 0.35
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 1.4
-
 2-keto-3-deoxy-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 4
-
 2-keto-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 25
-
 2-keto-3-deoxy-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.45 additional information
-
 additional information steady-state kinetics of the recombinant enzyme Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.13 Mg2+ required Cupriavidus necator
2.7.1.45 Mg2+ required Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.13 ATP + 2-dehydro-D-gluconate Cupriavidus necator
-
 ADP + 6-phospho-2-dehydro-D-gluconate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.13 Cupriavidus necator Q0K080
-
-
2.7.1.13 Cupriavidus necator Q0K080 i.e. Ralstonia eutropha strain H16
-
2.7.1.45 Cupriavidus necator Q0K080 i.e. Ralstonia eutropha strain H16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.13
-
 Cupriavidus necator
2.7.1.13 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and desalting gel filtration Cupriavidus necator
2.7.1.45 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and desalting gel filtration Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.13 8.7
-
 purified recombinant His-tagged enzyme, pH 8.0, 37°C Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.13 ATP + 2-dehydro-D-gluconate
-
 Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gluconate
-
 ?
2.7.1.13 ATP + 2-dehydro-D-gluconate
-
 Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gluconate
-
 r
2.7.1.13 ATP + 2-dehydro-D-gluconate preferred substrate Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gluconate
-
 r
2.7.1.13 ATP + 2-dehydro-D-gulonate
-
 Cupriavidus necator ADP + 6-phospho-2-dehydro-D-gulonate
-
 r
2.7.1.13 ATP + 2-keto-3-deoxy-D-gluconate
-
 Cupriavidus necator ADP + ?
-
 ?
2.7.1.13 ATP + 2-keto-3-deoxy-D-gulonate
-
 Cupriavidus necator ADP + ?
-
 ?
2.7.1.13 ATP + 2-keto-D-gulonate
-
 Cupriavidus necator ADP + ?
-
 ?
2.7.1.13 additional information the substrate specificity analysis reveals that three ketoacids (2-dehydro-3-deoxy-D-gluconate, 2-dehydro-D-gulonate, and 2-dehydro-3-deoxy-D-gulonate) with structures close to the natural substrate (2-dehydro-D-gluconate) are successfully phosphorylated at an efficiency lower than or comparable to 2-dehydrogluconate, as depicted by the measured kinetic constant values. Assay method optimization, overview Cupriavidus necator ?
-
-
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Cupriavidus necator ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 r
2.7.1.45 additional information the substrate specificity analysis reveals that three ketoacids (2-dehydro-3-deoxy-D-gluconate, 2-dehydro-D-gulonate, and 2-dehydro-3-deoxy-D-gulonate) with structures close to the natural substrate (2-dehydro-D-gluconate) are successfully phosphorylated at an efficiency lower than or comparable to 2-dehydrogluconate, as depicted by the measured kinetic constant values. Assay method optimization, overview Cupriavidus necator ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.13 ? x * 35700, recombinant His-tagged enzyme, SDS-PAGE Cupriavidus necator
2.7.1.45 ? x * 35700, recombinant His-tagged enzyme, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
2.7.1.13 2-dehydrogluconokinase
-
 Cupriavidus necator
2.7.1.13 2-ketogluconate kinase
-
 Cupriavidus necator
2.7.1.13 KGUK
-
 Cupriavidus necator
2.7.1.13 KGUKCnec
-
 Cupriavidus necator
2.7.1.13 More see also EC 2.7.1.45 Cupriavidus necator
2.7.1.45 2-dehydrogluconokinase
-
 Cupriavidus necator
2.7.1.45 2-ketogluconate kinase
-
 Cupriavidus necator
2.7.1.45 KGUK
-
 Cupriavidus necator
2.7.1.45 KGUKCnec
-
 Cupriavidus necator
2.7.1.45 More see also EC 2.7.1.13 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.13 37
-
 assay at Cupriavidus necator
2.7.1.45 37
-
 assay at Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.13 1.1
-
 2-keto-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 2.1
-
 2-keto-3-deoxy-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 5.71
-
 ATP pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 5.8
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 10.2
-
 2-keto-3-deoxy-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.13 8
-
 assay at Cupriavidus necator
2.7.1.45 8
-
 assay at Cupriavidus necator

General Information

EC Number General Information Comment Organism
2.7.1.13 physiological function 2-ketogluconate kinase (KGUK) is involved in the glucose and 2-ketogluconate catabolism of several aerobic bacteria, but relatively few bacterial species are able to utilize 2-ketogluconate as the sole carbon source for growth and energy provision Cupriavidus necator
2.7.1.45 physiological function 2-ketogluconate kinase (KGUK) is involved in the glucose and 2-ketogluconate catabolism of several aerobic bacteria, but relatively few bacterial species are able to utilize 2-ketogluconate as the sole carbon source for growth and energy provision Cupriavidus necator

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.13 0.083
-
 2-keto-3-deoxy-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 0.246
-
 2-keto-D-gulonate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 7.37
-
 2-keto-3-deoxy-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 16.77
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Cupriavidus necator
2.7.1.13 78.22
-
 ATP pH 8.0, temperature not specified in the publication Cupriavidus necator