EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.129 | gene pbp2A, recombinant expression of FLAG-tagged wild-type and mutant enzymes in Streptococcus pneumoniae strain D39 DELTAcps/DELTAmltG obtained from strain IU7260 | Streptococcus pneumoniae |
2.4.1.129 | gene penA or pbp2B, genotyping, recombinant expression in Streptococcus pneumoniae strain IU1824, i.e. D39 DELTAcps | Streptococcus pneumoniae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.129 | G494E | naturally occuring mutation, the mutant cannot be transformed with a DELTApbp2a deletion and shows the small-cell phenotype characteristic of DELTApbp1a mutants, reduced activity compared to wild-type | Streptococcus pneumoniae |
2.4.1.129 | additional information | construction of DELTA pbp2A deletion mutants. Domain architecture of PBP1a, TP active site motifs and mapped mutations in gene pbp1a in DELTAmltG suppressor strains. DELTApbp2A/DELTAmltG mutant phenotype, overview | Streptococcus pneumoniae |
2.4.1.129 | additional information | construction of DELTA pbp2B deletion mutants. Mutations in spd_1346 (mltG) suppress a DELTApbp2b deletion mutation. DELTApbp2B/DELTAmltG mutant phenotype, overview | Streptococcus pneumoniae |
2.4.1.129 | S89F | naturally occuring mutation, reduced activity compared to wild-type. The mutant can be transformed with a DELTApbp2a deletion. pbp1a(S89F) mutants shows an intermediate size | Streptococcus pneumoniae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.129 | membrane | - |
Streptococcus pneumoniae | 16020 | - |
2.4.1.129 | membrane | transmembrane protein | Streptococcus pneumoniae | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.129 | [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | Streptococcus pneumoniae | - |
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? | |
2.4.1.129 | [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | Streptococcus pneumoniae ATCC BAA-334 | - |
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.129 | Streptococcus pneumoniae | A0A0H2URT5 | - |
- |
2.4.1.129 | Streptococcus pneumoniae | P0A3M5 | - |
- |
2.4.1.129 | Streptococcus pneumoniae ATCC BAA-334 | A0A0H2URT5 | - |
- |
2.4.1.129 | Streptococcus pneumoniae ATCC BAA-334 | P0A3M5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.129 | [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Streptococcus pneumoniae | [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? | |
2.4.1.129 | [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Streptococcus pneumoniae ATCC BAA-334 | [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.129 | More | domain architecture of PBP1a | Streptococcus pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.129 | class A penicillin-binding protein | - |
Streptococcus pneumoniae |
2.4.1.129 | class B penicillin-binding protein | - |
Streptococcus pneumoniae |
2.4.1.129 | PBP2A | - |
Streptococcus pneumoniae |
2.4.1.129 | PBP2b | - |
Streptococcus pneumoniae |
2.4.1.129 | PenA | - |
Streptococcus pneumoniae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.129 | evolution | Streptococcus pneumoniae contains three class A (PBP1a, PBP2a and PBP1b) and two class B (PBP2x and PBP2b) enzymes. PBP1a and PBP2a are not equivalent | Streptococcus pneumoniae |
2.4.1.129 | malfunction | DELTAmltG mutants in unencapsulated strains accumulate inactivation mutations of class A PBP1a. The reduction of cell width and size of DELTApbp1a mutants compared to those of wild-type parent cells. Mutations in pbp1a suppress the DELTAmltG mutations | Streptococcus pneumoniae |
2.4.1.129 | malfunction | mutations that inactivate the pneumococcal YceG-domain protein, Spd_1346 (renamed MltG), remove the requirement for PBP2b. DELTAmltG mutants in unencapsulated strains accumulate inactivation mutations of class A PBP1a, which possesses TP and transglycosylase (TG) activities | Streptococcus pneumoniae |
2.4.1.129 | metabolism | the pneumococcal YceG-domain protein MltG releases anchored peptidoglycan (PG) glycan strands synthesized by PBP1a for crosslinking by a PBP2b:RodA complex in peripheral PG synthesis | Streptococcus pneumoniae |
2.4.1.129 | additional information | domain architecture of PBP1a | Streptococcus pneumoniae |
2.4.1.129 | additional information | the single MltG(Y488D) change suppresses the requirement for PBP2b, MreCD, RodZ and RodA | Streptococcus pneumoniae |
2.4.1.129 | physiological function | peptidoglycan (PG) is composed of glycan chains of beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) sugars that are crosslinked by PG peptides. Pneumococcal PG provides the major scaffold for the covalent attachment of wall-teichoic acid (WTA), capsule and surface proteins linked by sortases, many of which are virulence factors. Class A PBP1a, which possesses TP and transglycosylase (TG) activities | Streptococcus pneumoniae |
2.4.1.129 | physiological function | peptidoglycan (PG) is composed of glycan chains of beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) sugars that are crosslinked by PG peptides. Pneumococcal PG provides the major scaffold for the covalent attachment of wall-teichoic acid (WTA), capsule and surface proteins linked by sortases, many of which are virulence factors. In the pathogen Streptococcus pneumoniae (pneumococcus), side-wall (peripheral) peptidoglycan (PG) synthesis emanates from midcells and is catalyzed by the essential class B penicillin-binding protein PBP2b transpeptidase (TP). Class B PBP2x and PBP2b are essential for growth and required for septal and peripheral PG synthesis, respectively, in dividing Streptococcus pneumoniae cells. Pbp2b is essential in unencapsulated or encapsulated wild-type D39 strains | Streptococcus pneumoniae |