EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.23 | additional information | Derlin2 interacts with UBC6e in the complex involved in the regulation of ERAD enhancers. Derlin2 levels do not change in UBC6e-/- cells transduced with any of the UBC6e mutants, suggesting that it is not a substrate regulated by UBC6e, but is a member of a UBC6e complex | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.2.23 | additional information | generation of UbeC6 deletion mice, phenotype, detailed overview. Generation of Derlin2-/- mice showing that not only ERAD enhancers but also UBC6e itself are upregulated | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.2.23 | endoplasmic reticulum | UBC6e localizes to the ER via its tail-anchor, functional UBC6e requires its precise location in the endoplasmic reticulum to form a supramolecular complex with Derlin2 | Mus musculus | 5783 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.23 | S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine | Mus musculus | - |
[E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.23 | Mus musculus | Q9JJZ4 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.2.23 | embryonic fibroblast | MEFs | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.23 | S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine | - |
Mus musculus | [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.2.23 | More | UBC6e exists in at least two different configurations: as an apparent monomer and as part of a complex | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.2.23 | E2 Ub-conjugating enzyme | - |
Mus musculus |
2.3.2.23 | UBC6e | - |
Mus musculus |
2.3.2.23 | Ube2J1 | - |
Mus musculus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.2.23 | malfunction | ablation of UBC6e causes upregulation of active ERAD enhancers and so increases clearance not only of terminally misfolded substrates, but also of wild-type glycoproteins that fold comparatively slowly in vitro and in vivo. The absence of UBC6e increases the levels of ERAD enhancers with a corresponding increase in the rate of clearance of misfolded and/or incompletely folded substrates. UBC6e-/- MEFs show accelerated mannose trimming and premature substrate release from CNX, initiated by ER mannosidase-dependent eviction of substrate from the CNX cycle. Finally, by deletion of UBC6e, accelerated degradation is observed in tissue culture and in vivo, not only for canonical ERAD substrates, but also for folding intermediates of proteins that fold slowly, such as tyrosinase. The UBC6e loss-of-function mutation thus produces a gain-of-function with respect to ERAD activity, overview. Increased degradation of tyrosinase in UBC6e-/- cells and reduced skin tyrosinase levels in UBC6e-/- mice | Mus musculus |
2.3.2.23 | metabolism | the enzyme UbeC6 is involved in the endoplasmic reticulum (ER) associated degradation (ERAD) | Mus musculus |
2.3.2.23 | physiological function | UBC6e is an E2 ubiquitin conjugating enzyme that localizes to the ER via its tail-anchor. Functional UBC6e requires its precise location in the endoplasmic reticulum (ER) to form a supramolecular complex with Derlin2. This complex targets ERAD enhancers for degradation, a function that depends on UBC6e's enzymatic activity. UBC6e-/- cells upregulate ERAD enhancers selectively, but UBC6e downregulates EDEM1, EDEM3, OS-9 and SEL1L (ERAD enhancers) in the absence of ER stress. Homeostasis of SEL1L, EDEM1 and OS-9 requires enzymatic activity of UBC6e. UBC6e's function depends strictly on its E2 enzymatic activity but not its phosphorylation status. The exact ER membrane localization of UBC6e and the supramolecular complexes in which UBC6e participates determine its activity, and directly control the levels of components essential for ERAD activity. Only the Derlin2-complexed UBC6e controls the levels of ERAD enhancers | Mus musculus |