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Literature summary extracted from

  • Alarico, S.; Nunes-Costa, D.; Silva, A.; Costa, M.; Macedo-Ribeiro, S.; Empadinhas, N.
    A genuine mycobacterial thermophile Mycobacterium hassiacum growth, survival and GpgS stability at near-pasteurization temperatures (2020), Microbiology, 166, 474-483 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.266 expression in Escherichia coli Mycolicibacterium hassiacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.266 Mg2+ GpgS is dependent on divalent cations in the decreasing order of efficiency: Mg2+ (20 mM) > Mn2+ (10 mM) Mycolicibacterium hassiacum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.266 Mycolicibacterium hassiacum K5B7Z4
-
-
2.4.1.266 Mycolicibacterium hassiacum DSM 44199 K5B7Z4
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.266 0°C, one month, no loss of activity Mycolicibacterium hassiacum
2.4.1.266 23°C, one week, no loss of activity Mycolicibacterium hassiacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.266 ADP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 ADP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum DSM 44199 ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum DSM 44199 GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 additional information cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose Mycolicibacterium hassiacum ?
-
-
2.4.1.266 additional information cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose Mycolicibacterium hassiacum DSM 44199 ?
-
-
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium hassiacum DSM 44199 UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.266 ? x * 33600, calculated from sequence Mycolicibacterium hassiacum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.266 37
-
 half-life 7.8 days Mycolicibacterium hassiacum
2.4.1.266 50
-
 half-life 6.4 days Mycolicibacterium hassiacum
2.4.1.266 60
-
 5 min, loss of activity Mycolicibacterium hassiacum
2.4.1.266 63.5
-
 melting temperature, pH 7.5, in presence of substrates Mycolicibacterium hassiacum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.266 7 9
-
 Mycolicibacterium hassiacum