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Literature summary extracted from

  • Tauzin, A.; Bruel, L.; Laville, E.; Nicoletti, C.; Navarro, D.; Henrissat, B.; Perrier, J.; Potocki-Veronese, G.; Giardina, T.; Lafond, M.
    Sucrose 6F-phosphate phosphorylase A novel insight in the human gut microbiome (2019), Microb. Genom., 5, 1-14 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.329 expression in Escherichia coli [Ruminococcus] gnavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.329 1.7
-
 sucrose 6F-phosphate pH 6, 40°C [Ruminococcus] gnavus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.329 55000
-
 gel filtration [Ruminococcus] gnavus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.329 [Ruminococcus] gnavus G4T4R6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.329 alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
-
 [Ruminococcus] gnavus sucrose 6F-phosphate + phosphate
-
 r
2.4.1.329 additional information enzyme displays selective activity on sucrose 6F-phosphate acting both in phosphorolysis releasing alpha-D-glucose-1-phosphate and alpha-D-fructose-6-phosphate, and in reverse phosphorolysis from alpha-D-glucose-1-phosphate and alpha-D-fructose-6-phosphate to sucrose 6F-phosphate. No substrates: sucrose, D-fructose, D-glucose, D-galalctose, D-mannose, D-N-acetylgalactosamine, D-xylose, maltotetraose, maltoheptaose [Ruminococcus] gnavus ?
-
-
2.4.1.329 sucrose 6F-phosphate + phosphate
-
 [Ruminococcus] gnavus alpha-D-glucopyranose 1-phosphate + beta-D-fructofuranose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.329 monomer 1 * 55000, SDS-PAGE, 1 * 55165, calculated from sequence [Ruminococcus] gnavus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.329 RUGNEv3_61221 locus name [Ruminococcus] gnavus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.329 740
-
 sucrose 6F-phosphate pH 6, 40°C [Ruminococcus] gnavus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.329 435.3
-
 sucrose 6F-phosphate pH 6, 40°C [Ruminococcus] gnavus