EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.56 | synthesis | design of an in vivo NeuAc biosensor and application for genetic screening of a mutant library of NeuAc producers. A Ni2+-based selection system couples the cell growth with in vivo NeuAc concentration. The final strain produces up to 8.31 g/l NeuAc in minimal medium using glucose as sole carbon source | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.56 | A506G/G420A | increase in specific activity | Escherichia coli |
2.5.1.56 | C914T | decrease in specific activity | Escherichia coli |
2.5.1.56 | C914T/G1018A | no significant change in specific activity | Escherichia coli |
2.5.1.56 | G1018A | no significant change in specific activity | Escherichia coli |
2.5.1.56 | G283A | no significant change in specific activity | Escherichia coli |
2.5.1.56 | T74C | increase in specific activity | Escherichia coli |
2.5.1.56 | T74C/A506G | increase in specific activity | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.56 | Escherichia coli | - |
- |
- |
2.5.1.56 | Escherichia coli K1 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 17.93 | - |
mutant C914T, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 26.5 | - |
mutant G1018A, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 27.47 | - |
mutant C914T/G1018A, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 28.11 | - |
wild-type, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 28.96 | - |
mutant G283A, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 30.76 | - |
mutant T74C, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 32.48 | - |
mutant T74C/A506G, pH 7.0, 37°C | Escherichia coli |
2.5.1.56 | 35.71 | - |
mutant A506G/G420A, pH 7.0, 37°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.56 | phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | - |
Escherichia coli | phosphate + N-acetylneuraminate | - |
? | |
2.5.1.56 | phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O | - |
Escherichia coli K1 | phosphate + N-acetylneuraminate | - |
? |