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Literature summary extracted from

  • Nishiyama, R.; Inoue, A.; Ojima, T.
    Identification of 2-keto-3-deoxy-D-gluconate kinase and 2-keto-3-deoxy-D-phosphogluconate aldolase in an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01 (2017), Mar. Drugs, 15, 37 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.45 gene flkin, DNA and amino acid sequence determination and analysis, genetic organization of alginate-assimilating enzyme genes in the genome of Flavobacterium sp. strain UMI-01, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Flavobacterium sp. UMI-01

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.45 additional information construction of in vitro alginate-metabolizing system using recombinant enzymes. Alginate is allowed to react with recFlAlyA, recFlAlyB, recFlAlex, recFlRed, recFlKin, and recFlAld in various combinations at 25°C Flavobacterium sp. UMI-01

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.45 Mg2+ required Flavobacterium sp. UMI-01

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate Flavobacterium sp. UMI-01
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.45 Flavobacterium sp. UMI-01 A0A0B6VMB0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.45 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Flavobacterium sp. UMI-01

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.45 0.72
-
 purified recobinant enzyme, pH 7.4, 30°C Flavobacterium sp. UMI-01

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Flavobacterium sp. UMI-01 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.45 additional information thin-layer chromatography (TLC) and mass spectrometric analyses of reaction products of recombinant FlKin Flavobacterium sp. UMI-01 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.45 ? x * 39391, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE Flavobacterium sp. UMI-01

Synonyms

EC Number Synonyms Comment Organism
2.7.1.45 2-keto-3-deoxy-D-gluconate kinase
-
 Flavobacterium sp. UMI-01
2.7.1.45 FlKin
-
 Flavobacterium sp. UMI-01
2.7.1.45 KDG kinase
-
 Flavobacterium sp. UMI-01

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.45 50
-
 recombinant enzyme Flavobacterium sp. UMI-01

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.45 40
-
 purified recombinant enzyme, pH 7.0, 30 min, stable Flavobacterium sp. UMI-01

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.45 7
-
 recombinant enzyme Flavobacterium sp. UMI-01

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.45 7
-
 purified recombinant enzyme, 40°C, 30 min, stable Flavobacterium sp. UMI-01

General Information

EC Number General Information Comment Organism
2.7.1.45 evolution 2-keto-3-deoxy-D-gluconate (KDG) kinase and 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase genes, i.e., flkin and flald, are encoded in the assimilating gene cluster in the genome of Flavobacterium sp. strain UMI-01, a member of Bacteroidetes. The amino acid sequences deduced from flkin and flald show low identities with those of corresponding enzymes of Saccharophagus degradans strain 2-40T, a member of Proteobacteria Flavobacterium sp. UMI-01
2.7.1.45 metabolism the enzyme is active in alginate degradation. Unsaturated monosaccharide, the end product of alginate lyases, is spontaneously and/or enzymatically converted to an open chain form, DEH, and further converted to 2-keto-3-deoxy-D-gluconate (KDG) by the NAD(P)H-dependent DEH reductase. The KDG is phosphorylated to KDPG by KDG kinase and then split to pyruvate and glyceraldehyde-3-phosphate (GAP) by KDPG aldolase. The alginate-derived pyruvate and GAP are finally metabolized by Kreb's cycle. Alginate-metabolic system of Flavobacterium sp. strain UMI-01, overview Flavobacterium sp. UMI-01
2.7.1.45 physiological function the enzyme is active in alginate degradation Flavobacterium sp. UMI-01