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Literature summary extracted from

  • Li, L.; Wang, M.; Chen, Y.; Hu, T.; Yang, Y.; Zhang, Y.; Bi, G.; Wang, W.; Liu, E.; Han, J.; Lu, T.; Su, D.
    Structure of the enterovirus D68 RNA-dependent RNA polymerase in complex with NADPH implicates an inhibitor binding site in the RNA template tunnel (2020), J. Struct. Biol., 211, 107510 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expression in Escherichia coli BL21 (DE3) Enterovirus D68

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.48 sitting drop vapor diffusion method at 14°C, crystal structure of the enzyme (RdRp) in the apo state and in complex with the inhibitor NADPH Enterovirus D68

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 NADPH the enzyme activity decreases when titrated with NADPH in vitro Enterovirus A71
2.7.7.48 NADPH the inhibitor occupies the RNA template binding channel of Human enterovirus D68 enzyme (RdRp) with a binding pocket. Residues involved in the NADPH binding pocket of EV-D68 RdRp are highly conserved in RdRps of enteroviruses. The enzyme activity decreases when titrated with NADPH in vitro Enterovirus D68

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Enterovirus A71
-
 diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn Enterovirus D68
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Enterovirus A71
-
-
-
2.7.7.48 Enterovirus D68
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 Enterovirus A71
2.7.7.48
-
 Enterovirus D68

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Enterovirus A71 diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn
-
 Enterovirus D68 diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RDRP
-
 Enterovirus A71
2.7.7.48 RDRP
-
 Enterovirus D68
2.7.7.48 RNA-dependent RNA polymerase
-
 Enterovirus A71
2.7.7.48 RNA-dependent RNA polymerase
-
 Enterovirus D68

General Information

EC Number General Information Comment Organism
2.7.7.48 drug target the enzyme is considered as a promising target for specific antiviral therapeutics Enterovirus A71
2.7.7.48 drug target the enzyme is considered as a promising target for specific antiviral therapeutics Enterovirus D68
2.7.7.48 physiological function the enzyme is central for viral genome replication Enterovirus A71
2.7.7.48 physiological function the enzyme is central for viral genome replication Enterovirus D68