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Literature summary extracted from

  • Jin, M.; Huang, J.; Pei, Z.; Huang, J.; Gao, H.; Chang, Z.
    Purification and characterization of a high-salt-resistant microbial transglutaminase from Streptomyces mobaraensis (2016), J. Mol. Catal. B, 133, 6-11 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.3.2.13 food industry transglutaminase MTG-TX can be used in food-related applications in salty environment, e.g. bacon or seafood Streptomyces mobaraensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 Cu2+ strong inhibition Streptomyces mobaraensis
2.3.2.13 Fe3+ strong inhibition Streptomyces mobaraensis
2.3.2.13 additional information isozyme MTG-TX is not inhibited by Li+, Na+, K+, Ca2+, Mg2+, Ba2+, Mn2+, or the inhibitor phenylmethanesulfonyl fluoride (PMSF) Streptomyces mobaraensis
2.3.2.13 N-ethylmaleimide strong inhibition Streptomyces mobaraensis
2.3.2.13 Zn2+ strong inhibition Streptomyces mobaraensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 1.37
-
 hydroxylamine pH 6.0, 37°C Streptomyces mobaraensis
2.3.2.13 23.12
-
 N-CBZ-Glu-Gly pH 6.0, 37°C Streptomyces mobaraensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.13 extracellular
-
 Streptomyces mobaraensis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 additional information the enzyme is not affected by Cu2+ and Fe3+ Streptomyces mobaraensis
2.3.2.13 NaCl purification of a high-salt resistent microbial transglutaminase, isozyme MTG-TX, 79.8% activity remaining Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 protein glutamine + alkylamine Streptomyces mobaraensis
-
 protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine Streptomyces mobaraensis TX
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces mobaraensis P81453 collected from soil
-
2.3.2.13 Streptomyces mobaraensis TX P81453 collected from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 purification of extracellular high-salt resistent microbial transglutaminase, isozyme MTG-TX, by ethanol precipitation, cation exchange chromatography, hydrophobic interaction chromatography, and gel filtration, method optimization, overview Streptomyces mobaraensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.13 protein glutamine + alkylamine = protein N5-alkylglutamine + NH3 ping-pong mechanism Streptomyces mobaraensis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 39.2
-
 purified extracellular isozyme MTG-TX, pH 6.0, 37°C Streptomyces mobaraensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 N-CBZ-Glu-Gly + hydroxylamine
-
 Streptomyces mobaraensis CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3
-
 ?
2.3.2.13 N-CBZ-Glu-Gly + hydroxylamine
-
 Streptomyces mobaraensis TX CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine
-
 Streptomyces mobaraensis protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine
-
 Streptomyces mobaraensis TX protein N5-alkylglutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 37816, mass spectrometry Streptomyces mobaraensis

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 microbial transglutaminase
-
 Streptomyces mobaraensis
2.3.2.13 MTG-TX
-
 Streptomyces mobaraensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 48
-
 isozyme MTG-TX Streptomyces mobaraensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.13 20 70 activity range, profile overview Streptomyces mobaraensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.13 additional information
-
 the high-salt resistent isozyme MTG-TX exhibits a broad range of pH and temperature stability Streptomyces mobaraensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.13 1.83
-
 hydroxylamine pH 6.0, 37°C Streptomyces mobaraensis
2.3.2.13 1.83
-
 N-CBZ-Glu-Gly pH 6.0, 37°C Streptomyces mobaraensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 6
-
 isozyme MTG-TX Streptomyces mobaraensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.13 5 8 activity range of isozyme MTG-TX Streptomyces mobaraensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.13 5 10 the high-salt resistent isozyme MTG-TX exhibits a broad range of pH and temperature stability Streptomyces mobaraensis

General Information

EC Number General Information Comment Organism
2.3.2.13 additional information the enzyme shows a ping-pong mechanism Streptomyces mobaraensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.13 0.079
-
 N-CBZ-Glu-Gly pH 6.0, 37°C Streptomyces mobaraensis
2.3.2.13 1.34
-
 hydroxylamine pH 6.0, 37°C Streptomyces mobaraensis