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Literature summary extracted from

  • Nugroho, W.S.; Kim, D.W.; Han, J.C.; Hur, Y.B.; Nam, S.W.; Kim, H.J.
    Cloning, expression, and characterization of a cold-adapted shikimate kinase from the psychrophilic bacterium Colwellia psychrerythraea 34H (2016), J. Microbiol. Biotechnol., 26, 2087-2097 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71 expressed in Escherichia coli BL21(DE3) cells Colwellia psychrerythraea
2.7.1.71 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

General Stability

EC Number General Stability Organism
2.7.1.71 the urea concentration required for half-maximal denaturation is 1.5 mM Colwellia psychrerythraea
2.7.1.71 the urea concentration required for half-maximal denaturation is 2.5 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.71 0.1
-
 shikimate at pH 7.5 and 25°C Colwellia psychrerythraea
2.7.1.71 0.153
-
 ATP at pH 7.5 and 25°C Colwellia psychrerythraea
2.7.1.71 0.362
-
 shikimate at pH 7.5 and 25°C Escherichia coli
2.7.1.71 0.5
-
 ATP at pH 7.5 and 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ 15 mM used in assay conditions Colwellia psychrerythraea
2.7.1.71 Mg2+ 15 mM used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Colwellia psychrerythraea
-
 ADP + 3-phosphoshikimate
-
 r
2.7.1.71 ATP + shikimate Escherichia coli
-
 ADP + 3-phosphoshikimate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Colwellia psychrerythraea
-
-
-
2.7.1.71 Escherichia coli P0A6D7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.71 His-Bind agarose resin column chromatography Colwellia psychrerythraea
2.7.1.71 His-Bind agarose resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Colwellia psychrerythraea ADP + 3-phosphoshikimate
-
 r
2.7.1.71 ATP + shikimate
-
 Escherichia coli ADP + 3-phosphoshikimate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.71 ? x * about 20000, SDS-PAGE Colwellia psychrerythraea
2.7.1.71 ? x * about 20000, SDS-PAGE Escherichia coli
2.7.1.71 ? x * 19490, calculated from amino acid sequence Colwellia psychrerythraea

Synonyms

EC Number Synonyms Comment Organism
2.7.1.71 AroK
-
 Colwellia psychrerythraea
2.7.1.71 AroK
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.71 30
-
-
 Colwellia psychrerythraea
2.7.1.71 40
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.71 10 40 the enzyme retains about 40% of its maximal activity at 10°C and 40°C Colwellia psychrerythraea
2.7.1.71 15 40 the enzyme retains about 40% of its maximal activity at 15°C and 40°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.71 33
-
 melting temperature Colwellia psychrerythraea
2.7.1.71 45
-
 melting temperature Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.71 28.8
-
 shikimate at pH 7.5 and 25°C Colwellia psychrerythraea
2.7.1.71 32.8
-
 ATP at pH 7.5 and 25°C Colwellia psychrerythraea
2.7.1.71 55.86
-
 shikimate at pH 7.5 and 25°C Escherichia coli
2.7.1.71 122.51
-
 ATP at pH 7.5 and 25°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.71 154
-
 shikimate at pH 7.5 and 25°C Escherichia coli
2.7.1.71 214
-
 ATP at pH 7.5 and 25°C Colwellia psychrerythraea
2.7.1.71 245
-
 ATP at pH 7.5 and 25°C Escherichia coli
2.7.1.71 288
-
 shikimate at pH 7.5 and 25°C Colwellia psychrerythraea