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Literature summary extracted from

  • Maldonado, G.; Nava, G.; Plancarte, A.
    Two glutathione transferase isoforms isolated from juvenile cysts of Taenia crassiceps identification, purification and characterization (2018), J. Helminthol., 92, 687-695 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 bromosulfophthalein non-competitive inhibition Taenia crassiceps
2.5.1.18 Cibacron blue non-competitive inhibition Taenia crassiceps
2.5.1.18 haematin non-competitive inhibition Taenia crassiceps
2.5.1.18 indomethacin uncompetitive inhibition Taenia crassiceps
2.5.1.18 Rose bengal non-competitive inhibition Taenia crassiceps

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.3
-
 glutathione isoform GST2, at pH 7.5 and 25°C Taenia crassiceps
2.5.1.18 0.55
-
 glutathione isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 0.86
-
 1-chloro-2,4-dinitrobenzene isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.03
-
 1-chloro-2,4-dinitrobenzene isoform GST2, at pH 7.5 and 25°C Taenia crassiceps

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 54000
-
 isoform GST1, gel filtration Taenia crassiceps

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Taenia crassiceps
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 glutathione-Sepharose column chromatography Taenia crassiceps

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 cysticercus
-
 Taenia crassiceps
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 1.12
-
 isoform GST1, with trans-trans-2,4-nonadienal as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.27
-
 isoform GST2, with trans-trans-2,4-hexadienal as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.31
-
 isoform GST1, with trans-trans-2,4-hexadienal as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.4
-
 isoform GST2, with 1,2-dichloro-4,5-dinitrobenzene as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.49
-
 isoform GST2, with trans-trans-2,4-decadienal as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.51
-
 isoform GST2, with trans-trans-2,4-nonadienal as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 1.91
-
 isoform GST1, with trans-trans-2,4-decadienal as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 2
-
 isoform GST1, with 1,2-dichloro-4,5-dinitrobenzene as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 19.4
-
 isoform GST2, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 26.9
-
 isoform GST1, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 8.5 and 25°C Taenia crassiceps

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4,5-dinitrobenzene
-
 Taenia crassiceps ?
-
 ?
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Taenia crassiceps ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Taenia crassiceps chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + trans-trans-2,4-decadienal
-
 Taenia crassiceps ?
-
 ?
2.5.1.18 glutathione + trans-trans-2,4-hexadienal
-
 Taenia crassiceps ?
-
 ?
2.5.1.18 glutathione + trans-trans-2,4-nonadienal
-
 Taenia crassiceps ?
-
 ?
2.5.1.18 additional information bromosulfophthalein, 1,2-epoxy-3-(4-nitrophenoxy) propane and ethacrynic acid do not act as substrates Taenia crassiceps ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.18 homodimer 2 * 26800, isoform GST2, SDS-PAGE Taenia crassiceps
2.5.1.18 homodimer 2 * 27500, isoform GST1, SDS-PAGE Taenia crassiceps

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST1 isoform Taenia crassiceps
2.5.1.18 GST2 isoform Taenia crassiceps

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 35
-
 isoform GST1 Taenia crassiceps
2.5.1.18 40
-
 isoform GST2 Taenia crassiceps

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 20 55 isoform GST1 shows more than 80% activity between 20 and 55°C Taenia crassiceps
2.5.1.18 25 45 isoform GST2 shows more than 50% activity between 25 and 45°C Taenia crassiceps

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 20 45 the transferase activity of isoform GST2 is stable from 30 to 45°C but disappears quickly at higher temperatures. Additionally, the enzyme isoforms GST1 and GST2 are stable at 20°C and 37°C for 30 min Taenia crassiceps

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 1.99
-
 1-chloro-2,4-dinitrobenzene isoform GST2, at pH 7.5 and 25°C Taenia crassiceps
2.5.1.18 2.96
-
 glutathione isoform GST2, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 9.63
-
 glutathione isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 62.5
-
 1-chloro-2,4-dinitrobenzene isoform GST1, at pH 8.5 and 25°C Taenia crassiceps

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5
-
 isoform GST2 Taenia crassiceps
2.5.1.18 8.5
-
 isoform GST1 Taenia crassiceps

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.18 0.000508
-
 haematin isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 0.02
-
 indomethacin isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 0.3
-
 Cibacron blue isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 2.7
-
 Rose bengal isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 13
-
 bromosulfophthalein isoform GST1, at pH 8.5 and 25°C Taenia crassiceps

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00053
-
 isoform GST1, at pH 8.5 and 25°C Taenia crassiceps haematin
2.5.1.18 0.0009
-
 isoform GST2, at pH 8.5 and 25°C Taenia crassiceps Cibacron blue
2.5.1.18 0.0014
-
 isoform GST2, at pH 8.5 and 25°C Taenia crassiceps indomethacin
2.5.1.18 0.008
-
 isoform GST2, at pH 8.5 and 25°C Taenia crassiceps Rose bengal
2.5.1.18 0.0107
-
 isoform GST2, at pH 8.5 and 25°C Taenia crassiceps bromosulfophthalein
2.5.1.18 0.034
-
 isoform GST1, at pH 8.5 and 25°C Taenia crassiceps Rose bengal
2.5.1.18 0.2
-
 isoform GST1, at pH 8.5 and 25°C Taenia crassiceps indomethacin
2.5.1.18 1.6
-
 isoform GST1, at pH 8.5 and 25°C Taenia crassiceps bromosulfophthalein
2.5.1.18 2
-
 isoform GST1, at pH 8.5 and 25°C Taenia crassiceps Cibacron blue

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 1.9
-
 1-chloro-2,4-dinitrobenzene isoform GST2, at pH 7.5 and 25°C Taenia crassiceps
2.5.1.18 9.8
-
 glutathione isoform GST2, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 17.5
-
 glutathione isoform GST1, at pH 8.5 and 25°C Taenia crassiceps
2.5.1.18 72
-
 1-chloro-2,4-dinitrobenzene isoform GST1, at pH 8.5 and 25°C Taenia crassiceps