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Literature summary extracted from

  • Li, J.; Yu, L.; Li, J.; Xie, L.; Zhang, R.; Wang, H.
    Establishment of a high throughput-screening system for nucleoside deoxyribosyltransferase II mutant enzymes with altered substrate specificity (2019), J. Biosci. Bioeng., 128, 22-27 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.6 gene ndt, DNA and amino acid sequence determination and analysis, recombinant expression of enzyme NDT in Escherichia coli strain BL21(DE3), coexpression with gene cda encoding the cytidine deaminase from Escherichia coli Lactobacillus helveticus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.6 G10S site-directed mutagenesis, the NDT mutant displays improved activity for dideoxynucleoside Lactobacillus helveticus
2.4.2.6 additional information establishment of a site-saturation mutagenesis (SSM) library for LhNDT, analysis of enzyme-substrate interactions and amino acids near the NDT substrate-binding sites. Site-directed mutagenesis of resdieues Gly10, Ala11 and Leu96, high-throughput screening Lactobacillus helveticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Lactobacillus helveticus
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 2-deoxy-D-ribosyl-base2 + base1
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Lactobacillus helveticus Q8RLY4
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-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.6 0.13
-
 purified recombinant wild-type enzyme, pH 6.0, 37°C, substrate 2',3'-dideoxyinosine Lactobacillus helveticus
2.4.2.6 0.86
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 purified recombinant mutant NDT G10S, pH 6.0, 37°C, substrate 2',3'-dideoxyinosine Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2'-deoxyinosine + cytosine
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 Lactobacillus helveticus 2'-deoxycytidine + hypoxanthine
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 ?
2.4.2.6 2'-deoxythymidine + 5-azacytosine
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 Lactobacillus helveticus 5-aza-2'-deoxycytidine + thymine
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Lactobacillus helveticus 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 additional information NDT is highly specific for 20-deoxynucleoside because Glu98 of NDT forms a hydrogen bond with 3'-OH of 2'-deoxynucleoside. When the sugar donor is a ribonucleoside, Glu98 forms a hydrogen bond with 2'-OH of ribose and the enzyme is inactive. Usage of an indophenol colorimetric assay Lactobacillus helveticus ?
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Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 LhNDT
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 Lactobacillus helveticus
2.4.2.6 NDT
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 Lactobacillus helveticus
2.4.2.6 nucleoside deoxyribosyltransferase II
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 Lactobacillus helveticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.6 37
-
 assay at Lactobacillus helveticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.6 6
-
 assay at Lactobacillus helveticus

General Information

EC Number General Information Comment Organism
2.4.2.6 additional information homologous modeling submitting the three-dimensional structure of LlNDT (PDB ID 1F8X), and ligand interaction analysis, modeling of active site involved in the enzyme-substrate interaction Lactobacillus helveticus
2.4.2.6 physiological function nucleoside deoxyribosyltransferase II (NDT) catalyzes the transglycosylation reaction of the 2'-deoxyribose moiety between purine and/or pyrimidine bases Lactobacillus helveticus